Human IGFBP4 PicoKine® ELISA Kit

Insulin-like growth factor-binding protein 4 is a protein that in humans is encoded by the IGFBP4gene. This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma in both glycosylated and non-glycosylated forms. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. In addition, IGFBP-4 is a unique protein and it consistently inhibits several cancer cells in vivo and in vitro. Its inhibitory action has been shown in vivo in prostate and colon. It is secreted by all colon cancer cells.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 634 669 599 583 621 33.16 5.3% Sample 2 3283 3066 3571 3160 3270 190.05 5.8% Sample 3 15689 15202 16042 14388 15330 620.39 4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 634 669 599 583 621 33.16 5.3% Sample 1 634 669 599 583 621 33.16 5.3% Sample 2 3283 3066 3571 3160 3270 190.05 5.8% Sample 2 3283 3066 3571 3160 3270 190.05 5.8% Sample 3 15689 15202 16042 14388 15330 620.39 4% Sample 3 15689 15202 16042 14388 15330 620.39 4%

Angiogenesis, Cardiovascular, Cytoskeleton/ECM, Extracellular Matrix, Growth Factors, Growth Factors/Hormones, Lipid Signaling, Signal Transduction, Signaling Pathway, Structures

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 634 3283 15689 630 3596 16628 Standard deviation 29.16 164.15 957.02 40.32 222.95 1330.24 CV (%) 4.6% 5% 6.1% 6.4% 6.2% 8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 634 3283 15689 630 3596 16628 Standard deviation 29.16 164.15 957.02 40.32 222.95 1330.24 CV (%) 4.6% 5% 6.1% 6.4% 6.2% 8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 634 3283 15689 630 3596 16628 Mean (pg/ml) 634 3283 15689 630 3596 16628 Standard deviation 29.16 164.15 957.02 40.32 222.95 1330.24 Standard deviation 29.16 164.15 957.02 40.32 222.95 1330.24 CV (%) 4.6% 5% 6.1% 6.4% 6.2% 8% CV (%) 4.6% 5% 6.1% 6.4% 6.2% 8%

IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 500 1000 2000 4000 8000 16000 32000 O.D. 0.042 0.127 0.202 0.343 0.592 1.059 1.676 2.353 Concentration (pg/ml) 0 500 1000 2000 4000 8000 16000 32000 O.D. 0.042 0.127 0.202 0.343 0.592 1.059 1.676 2.353 Concentration (pg/ml) 0 500 1000 2000 4000 8000 16000 32000 Concentration (pg/ml) 0 500 1000 2000 4000 8000 16000 32000 O.D. 0.042 0.127 0.202 0.343 0.592 1.059 1.676 2.353 O.D. 0.042 0.127 0.202 0.343 0.592 1.059 1.676 2.353

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.