Human Activin A ELISA Kit PicoKine®

Activin A is a homodimer of 14kDa beta-A. Activin A, a cytokine member of the transforming growth factor-beta superfamily, is expressed locally by the mesenchymal component of the hemopoietic microenvironment. Its expression is regulated on the mRNA level by different cytokines, and the biological activity of the protein is tightly controlled by several inhibitory molecules. Inhibins and activins are members of the transforming growth factor beta superfamily and are known to modulate the growth and differentiation of several cell types. Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. The standard product used in this kit is recombinant Activin A, which is composed of two single chains of 116 amino acids with the molecular mass of 26KDa.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 34 40 35 33 35 2.69 7.6% Sample 2 199 204 220 225 212 10.79 5% Sample 3 467 440 464 464 458 10.89 2.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 34 40 35 33 35 2.69 7.6% Sample 1 34 40 35 33 35 2.69 7.6% Sample 2 199 204 220 225 212 10.79 5% Sample 2 199 204 220 225 212 10.79 5% Sample 3 467 440 464 464 458 10.89 2.3% Sample 3 467 440 464 464 458 10.89 2.3%

Inhibin, beta A (Activin A, activin AB alpha polypeptide) ; Inhibin, beta A (Activin A, activin AB alpha polypeptide), isoform CRA_a ; cDNA FLJ75379, highly similar to Homo sapiens inhibin, beta A (activin A, activin AB alpha polypeptide), mRNA ; INHBA ; hCG_17267 , tcag7.474

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 34 199 467 33 205 482 Standard deviation 1.63 15.72 29.88 2.54 14.76 37.96 CV (%) 4.8% 7.9% 6.4% 7.7% 7.2% 7.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 34 199 467 33 205 482 Standard deviation 1.63 15.72 29.88 2.54 14.76 37.96 CV (%) 4.8% 7.9% 6.4% 7.7% 7.2% 7.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 34 199 467 33 205 482 Mean (pg/ml) 34 199 467 33 205 482 Standard deviation 1.63 15.72 29.88 2.54 14.76 37.96 Standard deviation 1.63 15.72 29.88 2.54 14.76 37.96 CV (%) 4.8% 7.9% 6.4% 7.7% 7.2% 7.4% CV (%) 4.8% 7.9% 6.4% 7.7% 7.2% 7.4%

Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.031 0.112 0.17 0.291 0.469 0.823 1.376 2.027 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.031 0.112 0.17 0.291 0.469 0.823 1.376 2.027 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.031 0.112 0.17 0.291 0.469 0.823 1.376 2.027 O.D. 0.031 0.112 0.17 0.291 0.469 0.823 1.376 2.027

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.