Mouse MBL2/MPB-C ELISA Kit PicoKine®

MBL2, also called mannose-binding lectin (protein C) 2, soluble or Mannose-binding lectin (MBL) is a lectin that is instrumental in innate immunity. MBL2 is mapped to chromosome 10q11.2-q21. It belongs to the class of collectins in the C-type lectin superfamily, whose function appears to be pattern recognition in the first line of defense in the pre-immune host. MBL2 recognizes carbohydrate patterns, found on the surface of a large number of pathogenic micro-organisms, including bacteria, viruses, protozoa and fungi. Binding MBL2 to a micro-organism results in activation of the lectin pathway of the complement system. Another important function of MBL2 is that this molecule binds senescent and apoptotic cells and enhances engulfment of whole, intact apoptotic cells, as well as cell debris by phagocytes.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 656 711 654 663 671 23.33 3.4% Sample 2 1840 1861 1847 1853 1850 7.72 0.4% Sample 3 9232 10414 9072 9277 9498 533.88 5.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 656 711 654 663 671 23.33 3.4% Sample 1 656 711 654 663 671 23.33 3.4% Sample 2 1840 1861 1847 1853 1850 7.72 0.4% Sample 2 1840 1861 1847 1853 1850 7.72 0.4% Sample 3 9232 10414 9072 9277 9498 533.88 5.6% Sample 3 9232 10414 9072 9277 9498 533.88 5.6%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 656 1840 9232 637 1954 8997 Standard deviation 32.8 84.64 618.54 42.04 111.37 665.77 CV (%) 5% 4.6% 6.7% 6.6% 5.7% 7.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 656 1840 9232 637 1954 8997 Standard deviation 32.8 84.64 618.54 42.04 111.37 665.77 CV (%) 5% 4.6% 6.7% 6.6% 5.7% 7.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 656 1840 9232 637 1954 8997 Mean (pg/ml) 656 1840 9232 637 1954 8997 Standard deviation 32.8 84.64 618.54 42.04 111.37 665.77 Standard deviation 32.8 84.64 618.54 42.04 111.37 665.77 CV (%) 5% 4.6% 6.7% 6.6% 5.7% 7.4% CV (%) 5% 4.6% 6.7% 6.6% 5.7% 7.4%

Calcium-dependent lectin involved in innate immune defense. Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages (By similarity) . .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.006 0.086 0.146 0.313 0.533 0.993 1.745 2.284 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.006 0.086 0.146 0.313 0.533 0.993 1.745 2.284 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.006 0.086 0.146 0.313 0.533 0.993 1.745 2.284 O.D. 0.006 0.086 0.146 0.313 0.533 0.993 1.745 2.284

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.