Human MBL2/MPB-C ELISA Kit PicoKine®

Human MBL2/MPB-C ELISA Kit PicoKine® (96 Tests) . Quantitate Human MBL2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

MBL2, also called mannose-binding lectin (protein C) 2, soluble or Mannose-binding lectin (MBL) is a lectin that is instrumental in innate immunity. MBL2 is mapped to chromosome 10q11.2-q21. It belongs to the class of collectins in the C-type lectin superfamily, whose function appears to be pattern recognition in the first line of defense in the pre-immune host. MBL2 recognizes carbohydrate patterns, found on the surface of a large number of pathogenic micro-organisms, including bacteria, viruses, protozoa and fungi. Binding MBL2 to a micro-organism results in activation of the lectin pathway of the complement system. Another important function of MBL2 is that this molecule binds senescent and apoptotic cells and enhances engulfment of whole, intact apoptotic cells, as well as cell debris by phagocytes.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 479 546 475 475 493 30.21 6.1% Sample 2 2934 2910 2938 2814 2899 50.22 1.7% Sample 3 8702 9816 8527 9384 9107 519.57 5.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 479 546 475 475 493 30.21 6.1% Sample 1 479 546 475 475 493 30.21 6.1% Sample 2 2934 2910 2938 2814 2899 50.22 1.7% Sample 2 2934 2910 2938 2814 2899 50.22 1.7% Sample 3 8702 9816 8527 9384 9107 519.57 5.7% Sample 3 8702 9816 8527 9384 9107 519.57 5.7%

Synonyms

Mannose-binding protein C; MBP-C; Collectin-1; MBP1; Mannan-binding protein; Mannose-binding lectin; MBL2; COLEC1, MBL

Gene Name

MBL2

Gene ID

4153

UniProt

P11226

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: E21-I248

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Plasma protein produced mainly in the liver. .

Applications

ELISA

Field of Research

Complement, Immunology, Innate Immunity

Standard

20 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

312 pg/mL - 20,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 479 2854 8702 458 2785 8839 Standard deviation 27.3 129.09 661.35 34.35 147.6 742.47 CV (%) 5.7% 4.4% 7.6% 7.5% 5.3% 8.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 479 2854 8702 458 2785 8839 Standard deviation 27.3 129.09 661.35 34.35 147.6 742.47 CV (%) 5.7% 4.4% 7.6% 7.5% 5.3% 8.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 479 2854 8702 458 2785 8839 Mean (pg/ml) 479 2854 8702 458 2785 8839 Standard deviation 27.3 129.09 661.35 34.35 147.6 742.47 Standard deviation 27.3 129.09 661.35 34.35 147.6 742.47 CV (%) 5.7% 4.4% 7.6% 7.5% 5.3% 8.4% CV (%) 5.7% 4.4% 7.6% 7.5% 5.3% 8.4%

Sensitivity

<10 pg/mL

Function

Calcium-dependent lectin involved in innate immune defense. Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages. May bind DNA. .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

26144 MW

Specificity

Natural and recombinant human MBP-C

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Mannose-binding protein C

Subcellular Location

Secreted .

Sequence Similarities

Contains 1 C-type lectin domain.

Protein Name

Mannose-binding protein C

Sample Data

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.01 0.091 0.171 0.35 0.627 1.05 1.66 2.217 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.01 0.091 0.171 0.35 0.627 1.05 1.66 2.217 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.01 0.091 0.171 0.35 0.627 1.05 1.66 2.217 O.D. 0.01 0.091 0.171 0.35 0.627 1.05 1.66 2.217

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.