Human IGF2R/IGF-II R ELISA Kit PicoKine®

Human IGF2R/IGF-II R ELISA Kit PicoKine® (96 Tests) . Quantitate Human IGF2R in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Insulin-like growth factor 2 receptor, also called IGF2R or I-MPR is a protein that in humans is encoded by the IGF2R gene. This gene is mapped to 6q25.3. This gene encodes a receptor for both insulin-like growth factor 2 and mannose 6-phosphate, although the binding sites for either are located on different segments of the receptor. This receptor functions in the intracellular trafficking of lysosomal enzymes, the activation of transforming growth factor beta, and the degradation of insulin-like growth factor 2. While the related mouse gene shows exclusive expression from the maternal allele, imprinting of the human gene appears to be polymorphic, with only a minority of individuals showing expression from the maternal allele.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 249 245 232 224 237 10.01 4.2% Sample 2 1351 1344 1406 1445 1386 41.43 2.9% Sample 3 4462 4544 5035 4402 4610 250.07 5.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 249 245 232 224 237 10.01 4.2% Sample 1 249 245 232 224 237 10.01 4.2% Sample 2 1351 1344 1406 1445 1386 41.43 2.9% Sample 2 1351 1344 1406 1445 1386 41.43 2.9% Sample 3 4462 4544 5035 4402 4610 250.07 5.4% Sample 3 4462 4544 5035 4402 4610 250.07 5.4%

Synonyms

Cation-independent mannose-6-phosphate receptor; CI Man-6-P receptor; CI-MPR; M6PR;300 kDa mannose 6-phosphate receptor; MPR 300; Insulin-like growth factor 2 receptor; Insulin-like growth factor II receptor; IGF-II receptor; M6P/IGF2 receptor; M6P/IGF2R; CD222; IGF2R; MPRI

Gene Name

IGF2R

Gene ID

3482

UniProt

P11717

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: S1510-F2108

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Golgi Proteins, Immunology, Innate Immunity, Macrophage/Inflammation, Organelles, Protein Trafficking, Signal Transduction, Subcellular Markers

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 249 1351 4462 248 1314 4363 Standard deviation 9.96 77 197.32 13.64 94.42 253.05 CV (%) 4% 5.7% 4.4% 5.5% 7.3% 5.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 249 1351 4462 248 1314 4363 Standard deviation 9.96 77 197.32 13.64 94.42 253.05 CV (%) 4% 5.7% 4.4% 5.5% 7.3% 5.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 249 1351 4462 248 1314 4363 Mean (pg/ml) 249 1351 4462 248 1314 4363 Standard deviation 9.96 77 197.32 13.64 94.42 253.05 Standard deviation 9.96 77 197.32 13.64 94.42 253.05 CV (%) 4% 5.7% 4.4% 5.5% 7.3% 5.8% CV (%) 4% 5.7% 4.4% 5.5% 7.3% 5.8%

Sensitivity

<10 pg/mL

Function

Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6- phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. This receptor also binds IGF2. Acts as a positive regulator of T-cell coactivation, by binding DPP4. .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

274375 MW

Specificity

Natural and recombinant human IGF2R

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Cation-independent mannose-6-phosphate receptor

Subcellular Location

Lysosome membrane ; Single-pass type I membrane protein . Colocalized with DPP4 in internalized cytoplasmic vesicles adjacent to the cell surface.

Sequence Similarities

Belongs to the MRL1/IGF2R family.

Protein Name

Cation-independent mannose-6-phosphate receptor

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.061 0.13 0.193 0.292 0.586 0.966 1.594 2.053 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.061 0.13 0.193 0.292 0.586 0.966 1.594 2.053 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.061 0.13 0.193 0.292 0.586 0.966 1.594 2.053 O.D. 0.061 0.13 0.193 0.292 0.586 0.966 1.594 2.053

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.