Human ANGPTL3 ELISA Kit PicoKine®

ANGPTL3, also known as Angiopoietin-like 3 or HYPLl, is a protein that in humans is encoded by the ANGPTL3 gene. It is mapped to 1p31.3. The protein encoded by this gene is a member of the angiopoietin-like family of secreted factors. It is expressed predominantly in the liver, and has the characteristic structure of angiopoietins, consisting of a signal peptide, N-terminal coiled-coil domain, and the C-terminal fibrinogen (FBN) -like domain. ANGPTL3 also acts as dual inhibitor of lipoprotein lipase (LPL) and endothelial lipase (EL), and increases plasma triglyceride and HDL cholesterol in rodents. It can inhibit endothelial lipase to catalyze HDL-phospholipid and increase HDL-PL levels.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 704 723 638 687 688 31.55 4.5% Sample 2 1869 2168 2187 1992 2054 131.1 6.3% Sample 3 9766 8411 10192 8397 9191 801.78 8.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 704 723 638 687 688 31.55 4.5% Sample 1 704 723 638 687 688 31.55 4.5% Sample 2 1869 2168 2187 1992 2054 131.1 6.3% Sample 2 1869 2168 2187 1992 2054 131.1 6.3% Sample 3 9766 8411 10192 8397 9191 801.78 8.7% Sample 3 9766 8411 10192 8397 9191 801.78 8.7%

Angiogenesis, Cancer, Cardiovascular, Energy Metabolism, Energy Transfer Pathways, Growth Factors, Lipid Metabolism, Metabolic Signaling Pathways, Metabolism, Obesity, Pathways and Processes, Signal Transduction

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 704 1869 9766 766 1705 9664 Standard deviation 32.38 110.27 556.66 46.72 121.05 570.17 CV (%) 4.6% 5.7% 5.7% 6.1% 7.1% 5.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 704 1869 9766 766 1705 9664 Standard deviation 32.38 110.27 556.66 46.72 121.05 570.17 CV (%) 4.6% 5.7% 5.7% 6.1% 7.1% 5.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 704 1869 9766 766 1705 9664 Mean (pg/ml) 704 1869 9766 766 1705 9664 Standard deviation 32.38 110.27 556.66 46.72 121.05 570.17 Standard deviation 32.38 110.27 556.66 46.72 121.05 570.17 CV (%) 4.6% 5.7% 5.7% 6.1% 7.1% 5.9% CV (%) 4.6% 5.7% 5.7% 6.1% 7.1% 5.9%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.056 0.1 0.14 0.218 0.378 0.684 1.253 2.092 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.056 0.1 0.14 0.218 0.378 0.684 1.253 2.092 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.056 0.1 0.14 0.218 0.378 0.684 1.253 2.092 O.D. 0.056 0.1 0.14 0.218 0.378 0.684 1.253 2.092

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.