Rat CXCL3 ELISA Kit PicoKine®

Chemokine (C-X-C motif) ligand 3 (CXCL3) is a small cytokine belonging to the CXC chemokine family. It is mapped to 14p2 in rat. CXCL3 controls migration and adhesion of monocytes and mediates its effects on its target cell by interacting with a cell surface chemokine receptor. It has been shown that CXCL3 regulates cell autonomously the migration of the precursors of cerebellar granule neurons toward the internal layers of cerebellum, during the morphogenesis of cerebellum. CXCL3 also play fundamental roles in the development, homeostasis and it has effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 40 40 35 39 38 2.06 5.4% Sample 2 190 214 187 208 199 11.49 5.7% Sample 3 394 430 453 454 432 24.34 5.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 40 40 35 39 38 2.06 5.4% Sample 1 40 40 35 39 38 2.06 5.4% Sample 2 190 214 187 208 199 11.49 5.7% Sample 2 190 214 187 208 199 11.49 5.7% Sample 3 394 430 453 454 432 24.34 5.6% Sample 3 394 430 453 454 432 24.34 5.6%

C-X-C motif chemokine 3; Cytokine-induced neutrophil chemoattractant 2; CINC-2; Macrophage inflammatory protein 2-alpha/beta; MIP2-alpha/beta; Cxcl3; Cinc2

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 40 190 394 43 206 390 Standard deviation 2.84 14.82 19.3 3.09 19.98 20.28 CV (%) 7.1% 7.8% 4.9% 7.2% 9.7% 5.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 40 190 394 43 206 390 Standard deviation 2.84 14.82 19.3 3.09 19.98 20.28 CV (%) 7.1% 7.8% 4.9% 7.2% 9.7% 5.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 40 190 394 43 206 390 Mean (pg/ml) 40 190 394 43 206 390 Standard deviation 2.84 14.82 19.3 3.09 19.98 20.28 Standard deviation 2.84 14.82 19.3 3.09 19.98 20.28 CV (%) 7.1% 7.8% 4.9% 7.2% 9.7% 5.2% CV (%) 7.1% 7.8% 4.9% 7.2% 9.7% 5.2%

Ligand for CXCR2 (By similarity) . Has chemotactic activity for neutrophils. May play a role in inflammation and exert its effects on endothelial cells in an autocrine fashion. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.034 0.158 0.246 0.402 0.6253 1.099 1.701 2.314 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.034 0.158 0.246 0.402 0.6253 1.099 1.701 2.314 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.034 0.158 0.246 0.402 0.6253 1.099 1.701 2.314 O.D. 0.034 0.158 0.246 0.402 0.6253 1.099 1.701 2.314

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.