Human ADAMTS4 ELISA Kit PicoKine®

Human ADAMTS4 ELISA Kit PicoKine® (96 Tests) . Quantitate Human ADAMTS4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 20pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

ADAMTS4, A disintegrin and metalloproteinase with thrombospondin motifs 4 is an enzyme that in humans is encoded by the ADAMTS4 gene. ADAMTS4 is a member of the large ADAMTS family of zinc-dependent proteases. The human ADAMTS4 gene is mapped to chromosome 1 by somatic cell hybrid analysis. The enzyme encoded by this gene lacks a C-terminal TS motif. It is responsible for the degradation of aggrecan, a major proteoglycan of cartilage, and brevican, a brain-specific extracellular matrix protein. The cleavage of aggrecan and brevican suggests key roles of this enzyme in arthritic disease and in the central nervous system, potentially, in the progression of glioma.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1127 1186 1062 1229 1151 62.86 5.4% Sample 2 4290 4643 4014 4511 4364 238.44 5.4% Sample 3 29063 29288 30434 26599 28846 1397.6 4.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1127 1186 1062 1229 1151 62.86 5.4% Sample 1 1127 1186 1062 1229 1151 62.86 5.4% Sample 2 4290 4643 4014 4511 4364 238.44 5.4% Sample 2 4290 4643 4014 4511 4364 238.44 5.4% Sample 3 29063 29288 30434 26599 28846 1397.6 4.8% Sample 3 29063 29288 30434 26599 28846 1397.6 4.8%

Synonyms

A disintegrin and metalloproteinase with thrombospondin motifs 4; ADAM-TS 4; ADAM-TS4; ADAMTS-4;3.4.24.82; ADMP-1; Aggrecanase-1; ADAMTS4; KIAA0688; UNQ769/PRO1563

Gene Name

ADAMTS4

Gene ID

9507

UniProt

O75173

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: CHO; Immunogen sequence: F213-C685

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Expressed in brain, lung and heart. Expressed at very low level in placenta and skeletal muscles. Isoform 2 is detected in osteoarthritic synovium. .

Applications

ELISA

Field of Research

Cancer, Cell Biology, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Invasion/Microenvironment, Metalloprotease, Proteolysis/Ubiquitin, Proteolytic Enzymes, Signal Transduction

Standard

40 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

625 pg/mL - 40,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1127 4290 29063 1210 4672 29313 Standard deviation 46.2 184.47 1627.52 71.39 200.89 2081.22 CV (%) 4.1% 4.3% 5.6% 5.9% 4.3% 7.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1127 4290 29063 1210 4672 29313 Standard deviation 46.2 184.47 1627.52 71.39 200.89 2081.22 CV (%) 4.1% 4.3% 5.6% 5.9% 4.3% 7.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1127 4290 29063 1210 4672 29313 Mean (pg/ml) 1127 4290 29063 1210 4672 29313 Standard deviation 46.2 184.47 1627.52 71.39 200.89 2081.22 Standard deviation 46.2 184.47 1627.52 71.39 200.89 2081.22 CV (%) 4.1% 4.3% 5.6% 5.9% 4.3% 7.1% CV (%) 4.1% 4.3% 5.6% 5.9% 4.3% 7.1%

Sensitivity

<20 pg/mL

Function

Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. Could also be a critical factor in the exacerbation of neurodegeneration in Alzheimer disease. Cleaves aggrecan at the '392-Glu-|-Ala-393' site.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

90197 MW

Specificity

Natural and recombinant human ADAMTS4

Anticoagulant

Heparin or EDTA

Gene Name Synonym

A disintegrin and metalloproteinase with thrombospondin motifs 4

Subcellular Location

Secreted, extracellular space, extracellular matrix .

Sequence Similarities

Contains 1 disintegrin domain.

Protein Name

A disintegrin and metalloproteinase with thrombospondin motifs 4

Sample Data

Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 O.D. 0.064 0.16 0.222 0.308 0.522 0.921 1.448 2.465 Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 O.D. 0.064 0.16 0.222 0.308 0.522 0.921 1.448 2.465 Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 O.D. 0.064 0.16 0.222 0.308 0.522 0.921 1.448 2.465 O.D. 0.064 0.16 0.222 0.308 0.522 0.921 1.448 2.465

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.