Mouse Hemojuvelin/RGM-C/HJV ELISA Kit PicoKine®

Hemojuvelin (HJV/RGMc/HFE2) is a membrane-bound and soluble protein in mammals. It is a member of a three gene family (in vertebrates) called the repulsive guidance molecules. In mouse, the Hemojuvelin protein is encoded by the HFE2 gene, and it is mapped to Chromosome 3. It was concluded that downregulation of hepatic Hemojuvelin during inflammation may induce a temporary elimination of iron sensing. Hemojuvelin is a coreceptor for bone morphogenetic proteins (BMPs), and inhibition of endogenous BMP signaling reduces hepcidin expression and increases serum iron in mice. Furthermore, Hemojuvelin may play inhibitory roles in prostate cancer by suppressing cell growth, adhesion, migration and invasion.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 174 203 203 206 196 13.04 6.6% Sample 2 404 461 449 447 440 21.6 4.9% Sample 3 1769 1736 1928 1768 1800 74.94 4.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 174 203 203 206 196 13.04 6.6% Sample 1 174 203 203 206 196 13.04 6.6% Sample 2 404 461 449 447 440 21.6 4.9% Sample 2 404 461 449 447 440 21.6 4.9% Sample 3 1769 1736 1928 1768 1800 74.94 4.1% Sample 3 1769 1736 1928 1768 1800 74.94 4.1%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 174 404 1769 170 398 1835 Standard deviation 11.48 18.98 714.453 14.45 25.87 114.7% CV (%) 6.6% 4.7% 4.4% 8.5% 6.5% 6.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 174 404 1769 170 398 1835 Standard deviation 11.48 18.98 714.453 14.45 25.87 114.7% CV (%) 6.6% 4.7% 4.4% 8.5% 6.5% 6.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 174 404 1769 170 398 1835 Mean (pg/ml) 174 404 1769 170 398 1835 Standard deviation 11.48 18.98 714.453 14.45 25.87 114.7% Standard deviation 11.48 18.98 714.453 14.45 25.87 114.7% CV (%) 6.6% 4.7% 4.4% 8.5% 6.5% 6.3% CV (%) 6.6% 4.7% 4.4% 8.5% 6.5% 6.3%

Acts as a bone morphogenetic protein (BMP) coreceptor. Through enhancement of BMP signaling regulates hepcidin (HAMP) expression and regulates iron homeostasis. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.033 0.13 0.219 0.279 0.5 0.814 1.246 1.919 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.033 0.13 0.219 0.279 0.5 0.814 1.246 1.919 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.033 0.13 0.219 0.279 0.5 0.814 1.246 1.919 O.D. 0.033 0.13 0.219 0.279 0.5 0.814 1.246 1.919

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.