Mouse Tissue Factor/F3 ELISA Kit PicoKine®

Tissue factor, also called platelet tissue factor, factor III, thrombokinase, or CD142 is a protein present in sub endothelial tissue and leukocytes necessary for the initiation of thrombin formation from the zymogene prothrombin. Tissue factor gene was mapped to 1pter-p21 by study of somatic cell hybrids with a species-specific sensitive chromogenic assay. This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 31 36 32 30 32 2.27 7% Sample 2 167 171 178 166 170 4.71 2.7% Sample 3 444 389 387 433 413 25.55 6.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 31 36 32 30 32 2.27 7% Sample 1 31 36 32 30 32 2.27 7% Sample 2 167 171 178 166 170 4.71 2.7% Sample 2 167 171 178 166 170 4.71 2.7% Sample 3 444 389 387 433 413 25.55 6.1% Sample 3 444 389 387 433 413 25.55 6.1%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 31 167 444 32 169 432 Standard deviation 1.3 9.18 32.41 1.5 10.47 35.85 CV (%) 4.2% 5.5% 7.3% 4.7% 6.2% 8.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 31 167 444 32 169 432 Standard deviation 1.3 9.18 32.41 1.5 10.47 35.85 CV (%) 4.2% 5.5% 7.3% 4.7% 6.2% 8.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 31 167 444 32 169 432 Mean (pg/ml) 31 167 444 32 169 432 Standard deviation 1.3 9.18 32.41 1.5 10.47 35.85 Standard deviation 1.3 9.18 32.41 1.5 10.47 35.85 CV (%) 4.2% 5.5% 7.3% 4.7% 6.2% 8.3% CV (%) 4.2% 5.5% 7.3% 4.7% 6.2% 8.3%

Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.017 0.064 0.127 0.248 0.48 0.822 1.297 2.024 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.017 0.064 0.127 0.248 0.48 0.822 1.297 2.024 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.017 0.064 0.127 0.248 0.48 0.822 1.297 2.024 O.D. 0.017 0.064 0.127 0.248 0.48 0.822 1.297 2.024

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.