Human IL-34/Interleukin-34 ELISA Kit PicoKine®

Interleukin-34, or IL-34 is a protein belonging to a group of cytokines called interleukins. It is mapped to 16q22.1. The discovery of IL-34 protein in the red pulp of the spleen suggests involvement in growth and development of myeloid cells, consistent with its activity on monocytes. IL-34 is a recently discovered cytokine that acts on tissue resident macrophages and Langerhans cells upon binding the receptor for CSF-1, CSF-1R. It also can promote the differentiation and viability of monocytes and macrophages through the colony-stimulating factor-1 receptor. In addition, it promotes the development, survival, and function of microglia and Langerhans cells, therefore, this cytokine may predominately function in brain and skin biology.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 93 84 90 91 89 3.35 3.7% Sample 2 548 521 516 487 518 21.64 4.1% Sample 3 1157 1352 1188 1232 1232 74.09 6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 93 84 90 91 89 3.35 3.7% Sample 1 93 84 90 91 89 3.35 3.7% Sample 2 548 521 516 487 518 21.64 4.1% Sample 2 548 521 516 487 518 21.64 4.1% Sample 3 1157 1352 1188 1232 1232 74.09 6% Sample 3 1157 1352 1188 1232 1232 74.09 6%

Detected in the sinusoidal epithelium in the red pulp of spleen (at protein level) . Predominantly expressed in spleen. Also detected in a range of other tissues including heart, brain, lung, liver, kidney, thymus, testis, ovary, small intestine, prostate and colon. .

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 93 548 1157 96 552 1148 Standard deviation 5.3 33.42 67.1 5.85 35.32 73.47 CV (%) 5.7% 6.1% 5.8% 6.1% 6.4% 6.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 93 548 1157 96 552 1148 Standard deviation 5.3 33.42 67.1 5.85 35.32 73.47 CV (%) 5.7% 6.1% 5.8% 6.1% 6.4% 6.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 93 548 1157 96 552 1148 Mean (pg/ml) 93 548 1157 96 552 1148 Standard deviation 5.3 33.42 67.1 5.85 35.32 73.47 Standard deviation 5.3 33.42 67.1 5.85 35.32 73.47 CV (%) 5.7% 6.1% 5.8% 6.1% 6.4% 6.4% CV (%) 5.7% 6.1% 5.8% 6.1% 6.4% 6.4%

Cytokine that promotes the proliferation, survival and differentiation of monocytes and macrophages. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, and in the regulation of bone resorption. Signaling via CSF1R and its downstream effectors stimulates phosphorylation of MAPK1/ERK2 AND MAPK3/ERK1. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.077 0.161 0.212 0.322 0.55 1.064 1.694 2.086 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.077 0.161 0.212 0.322 0.55 1.064 1.694 2.086 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.077 0.161 0.212 0.322 0.55 1.064 1.694 2.086 O.D. 0.077 0.161 0.212 0.322 0.55 1.064 1.694 2.086

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.