Mouse IL-17D ELISA Kit PicoKine®

Mouse IL-17D ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Il17d in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Interleukin 17D (IL-17D) is a member of the unique IL-17 family, which share a conserved cysteine motif. It is mapped to 13q12.11. IL-17D regulates cytokine production in endothelial cells and inhibits hemopoiesis in vitro. Purified recombinant IL-17D inhibited myeloid progenitor cell colony formation. IL-17D can enhance endothelial cell production of IL6, IL8, and GMCSF, but not other cytokines. And it is preferentially expressed in skeletal muscle, brain, adipose tissue, heart, lung, and pancreas.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 147 150 156 137 147 6.87 4.6% Sample 2 775 677 680 767 724 46.34 6.4% Sample 3 1330 1370 1170 1232 1275 78.93 6.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 147 150 156 137 147 6.87 4.6% Sample 1 147 150 156 137 147 6.87 4.6% Sample 2 775 677 680 767 724 46.34 6.4% Sample 2 775 677 680 767 724 46.34 6.4% Sample 3 1330 1370 1170 1232 1275 78.93 6.1% Sample 3 1330 1370 1170 1232 1275 78.93 6.1%

Synonyms

IL-17D ; Il17d

Gene Name

IL17D

Gene ID

239114

UniProt

Q8K4C4

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A25-R205

Capture Antibody

Monoclonal antibody from rat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Cancer, Cytokines, Immunology, Innate Immunity, Interleukins, Tumor Immunology

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

62.5 pg/mL - 4,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 147 775 1330 157 700 1222 Standard deviation 5.88 51.15 98.42 8.79 50.4 97.76 CV (%) 4% 6.6% 7.4% 5.6% 7.2% 8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 147 775 1330 157 700 1222 Standard deviation 5.88 51.15 98.42 8.79 50.4 97.76 CV (%) 4% 6.6% 7.4% 5.6% 7.2% 8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 147 775 1330 157 700 1222 Mean (pg/ml) 147 775 1330 157 700 1222 Standard deviation 5.88 51.15 98.42 8.79 50.4 97.76 Standard deviation 5.88 51.15 98.42 8.79 50.4 97.76 CV (%) 4% 6.6% 7.4% 5.6% 7.2% 8% CV (%) 4% 6.6% 7.4% 5.6% 7.2% 8%

Sensitivity

<10 pg/mL

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

22391 MW

Specificity

Natural and recombinant mouse IL-17D

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Interleukin 17D

Protein Name

Interleukin-17D

Sample Data

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.004 0.142 0.197 0.316 0.586 0.984 1.446 2.096 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.004 0.142 0.197 0.316 0.586 0.984 1.446 2.096 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.004 0.142 0.197 0.316 0.586 0.984 1.446 2.096 O.D. 0.004 0.142 0.197 0.316 0.586 0.984 1.446 2.096

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.