Human CEACAM1/Cd66A ELISA Kit PicoKine®

CEACAM1, also known as CD66a (Cluster of Differentiation 66a), is a human glycoprotein encoded by a gene. This gene encodes a member of the carcinoembryonic antigen (CEA) gene family, which belongs to the immunoglobulin superfamily. CEACAM1 is mapped to 19q13.2 in human. The encoded protein was originally described in bile ducts of liver as biliary glycoprotein. Subsequently, it was found to be a cell-cell adhesion molecule detected on leukocytes, epithelia, and endothelia. The encoded protein mediates cell adhesion via homophilic as well as heterophilic binding to other proteins of the subgroup. Multiple cellular activities have been attributed to the encoded protein, including roles in the differentiation and arrangement of tissue three-dimensional structure, angiogenesis, apoptosis, tumor suppression, metastasis, and the modulation of innate and adaptive immune responses.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 463 407 434 425 432 20.24 4.6% Sample 2 2835 2793 2564 2668 2715 106.64 3.9% Sample 3 9439 8061 8668 8372 8635 511.4 5.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 463 407 434 425 432 20.24 4.6% Sample 1 463 407 434 425 432 20.24 4.6% Sample 2 2835 2793 2564 2668 2715 106.64 3.9% Sample 2 2835 2793 2564 2668 2715 106.64 3.9% Sample 3 9439 8061 8668 8372 8635 511.4 5.9% Sample 3 9439 8061 8668 8372 8635 511.4 5.9%

Cancer, Cell Type Markers, Human Protein, Microbiology, Oncoproteins/Suppressors, Protein, Tags & Cell Markers, Tumor Antigens, Tumor Associated, Tumor Biomarkers, Tumor Immunology, Tumor Suppressors, Tumor-Associated Antigens

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 463 2835 9439 432 3010 9810 Standard deviation 32.41 150.25 453.07 35.42 207.69 529.74 CV (%) 7% 5.3% 4.8% 8.2% 6.9% 5.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 463 2835 9439 432 3010 9810 Standard deviation 32.41 150.25 453.07 35.42 207.69 529.74 CV (%) 7% 5.3% 4.8% 8.2% 6.9% 5.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 463 2835 9439 432 3010 9810 Mean (pg/ml) 463 2835 9439 432 3010 9810 Standard deviation 32.41 150.25 453.07 35.42 207.69 529.74 Standard deviation 32.41 150.25 453.07 35.42 207.69 529.74 CV (%) 7% 5.3% 4.8% 8.2% 6.9% 5.4% CV (%) 7% 5.3% 4.8% 8.2% 6.9% 5.4%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.032 0.088 0.143 0.248 0.423 0.816 1.359 2.031 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.032 0.088 0.143 0.248 0.423 0.816 1.359 2.031 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.032 0.088 0.143 0.248 0.423 0.816 1.359 2.031 O.D. 0.032 0.088 0.143 0.248 0.423 0.816 1.359 2.031

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.