Human TFPI2 ELISA Kit PicoKine®

TFPI2, also called Tissue factor pathway inhibitor 2, is a protein that in humans is encoded by the TFPI2 gene. It is mapped to 7q21.3. This gene encodes a member of the Kunitz-type serine proteinase inhibitor family. The protein can inhibit a variety of serine proteases including factor VIIa/tissue factor, factor Xa, plasmin, trypsin, chymotryspin and plasma kallikrein. This gene has been identified as a tumor suppressor gene in several types of cancer. Qualitative PCR and microarray analyses showed that expression of TFPI2 was inversely correlated with that of MIR616 in prostate cancer cell lines, primary tumors of various grades, and normal tissue.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 143 191 141 241 179 5.96 3.3% Sample 2 811 946 836 757 838 62.98 7.5% Sample 3 2856 2959 2892 3245 2988 154.78 5.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 143 191 141 241 179 5.96 3.3% Sample 1 143 191 141 241 179 5.96 3.3% Sample 2 811 946 836 757 838 62.98 7.5% Sample 2 811 946 836 757 838 62.98 7.5% Sample 3 2856 2959 2892 3245 2988 154.78 5.2% Sample 3 2856 2959 2892 3245 2988 154.78 5.2%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 143 811 2856 190 797 3495 Standard deviation 7.56 48.5 130.52 10.30 33.16 89.47 CV (%) 5.3% 6.0% 4.6% 5.4% 4.2% 2.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 143 811 2856 190 797 3495 Standard deviation 7.56 48.5 130.52 10.30 33.16 89.47 CV (%) 5.3% 6.0% 4.6% 5.4% 4.2% 2.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 143 811 2856 190 797 3495 Mean (pg/ml) 143 811 2856 190 797 3495 Standard deviation 7.56 48.5 130.52 10.30 33.16 89.47 Standard deviation 7.56 48.5 130.52 10.30 33.16 89.47 CV (%) 5.3% 6.0% 4.6% 5.4% 4.2% 2.6% CV (%) 5.3% 6.0% 4.6% 5.4% 4.2% 2.6%

May play a role in the regulation of plasmin-mediated matrix remodeling. Inhibits trypsin, plasmin, factor VIIa/tissue factor and weakly factor Xa. Has no effect on thrombin. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 125 250 500 1000 2000 4000 O.D. 0.031 0.257 0.471 0.84 1.389 2.02 2.386 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 O.D. 0.031 0.257 0.471 0.84 1.389 2.02 2.386 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 O.D. 0.031 0.257 0.471 0.84 1.389 2.02 2.386 O.D. 0.031 0.257 0.471 0.84 1.389 2.02 2.386

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.