Human Neuropilin-1 ELISA Kit PicoKine®

NRP1 (Neuropilin 1) also known as NP1, NRP, BDCA4 or VEGF165R, is a membrane-bound coreceptor to a tyrosine kinase receptor for both vascular endothelial growth factor (VEGF) and semaphorin family members. NRP1 plays versatile roles in angiogenesis, axon guidance, cell survival, migration, and invasion. By somatic cell hybrid analysis, the NRP1 gene was mapped to chromosome 10. NRP1 bounds PGF1 with lower affinity. NRP1-mediated interactions are a necessary element in the initiation of the primary immune response and offer another example, like that of agrin, of a molecule shared by neurologic and immunologic synapses. After T-cell contact with DC, T-cell NRP1 colocalized with CD3 in the immunologic synapse and, sometimes, also at the opposite pole of the T cell. Soluble NRP1 interacts in a homophilic fashion with NRP1 on both DC and T cells, and this binding can be inhibited by blocking antibodies to NRP1. Furthermore, selective NRP1 inhibition in this model suppressed neovascular formation substantially.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1578 1405 1534 1693 1552 103.06 6.6% Sample 2 4783 4689 4619 4324 4603 171.67 3.7% Sample 3 20934 22048 20450 19219 20662 1015.15 4.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1578 1405 1534 1693 1552 103.06 6.6% Sample 1 1578 1405 1534 1693 1552 103.06 6.6% Sample 2 4783 4689 4619 4324 4603 171.67 3.7% Sample 2 4783 4689 4619 4324 4603 171.67 3.7% Sample 3 20934 22048 20450 19219 20662 1015.15 4.9% Sample 3 20934 22048 20450 19219 20662 1015.15 4.9%

The expression of isoforms 1 and 2 does not seem to overlap. Isoform 1 is expressed by the blood vessels of different tissues. In the developing embryo it is found predominantly in the nervous system. In adult tissues, it is highly expressed in heart and placenta; moderately in lung, liver, skeletal muscle, kidney and pancreas; and low in adult brain. Isoform 2 is found in liver hepatocytes, kidney distal and proximal tubules.

Adaptive Immunity, Angiogenesis, Axonal Guidance Proteins, Cardiovascular, Growth and Development, Growth Factors, Immunology, Neurology Process, Neuroscience

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1578 4783 20934 1501 4697 20813 Standard deviation 69.43 316.67 921.09 90.06 371.06 1207.15 CV (%) 4.4% 6.6% 4.4% 6% 7.9% 5.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1578 4783 20934 1501 4697 20813 Standard deviation 69.43 316.67 921.09 90.06 371.06 1207.15 CV (%) 4.4% 6.6% 4.4% 6% 7.9% 5.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1578 4783 20934 1501 4697 20813 Mean (pg/ml) 1578 4783 20934 1501 4697 20813 Standard deviation 69.43 316.67 921.09 90.06 371.06 1207.15 Standard deviation 69.43 316.67 921.09 90.06 371.06 1207.15 CV (%) 4.4% 6.6% 4.4% 6% 7.9% 5.8% CV (%) 4.4% 6.6% 4.4% 6% 7.9% 5.8%

The membrane-bound isoform 1 is a receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. It mediates the chemorepulsant activity of semaphorins. It binds to semaphorin 3A, The PLGF-2 isoform of PGF, The VEGF-165 isoform of VEGF and VEGF-B. Coexpression with KDR results in increased VEGF-165 binding to KDR as well as increased chemotaxis. It may regulate VEGF-induced angiogenesis.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.046 0.105 0.159 0.249 0.453 0.84 1.483 2.062 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.046 0.105 0.159 0.249 0.453 0.84 1.483 2.062 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.046 0.105 0.159 0.249 0.453 0.84 1.483 2.062 O.D. 0.046 0.105 0.159 0.249 0.453 0.84 1.483 2.062

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.