Human SP-D / Surfactant protein D ELISA Kit PicoKine®

Human SP-D / Surfactant protein D ELISA Kit PicoKine® (96 Tests) . Quantitate Human SFTPD in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 20pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Surfactant, pulmonary-associated protein D, also known as SFTPD or SP-D, is a protein which in humans is encoded by the SFTPD gene. By fluorescence in situ hybridization, the SP-D gene was localized in 10q22.2-q23.1. On the basis of homology with other collectins, potential functions for SP-D include roles in innate immunity and surfactant metabolism, SP-D is produced in the bronchiolar and terminal epithelium of human fetal lung from about 21 weeks of gestation. What’s more, SP-A and SP-D act as dual-function surveillance molecules that reverse orientation and function and become initiators of host-defense reactions.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1547 1291 1390 1463 1422 94.19 6.6% Sample 2 3580 3482 3689 3752 3625 103.31 2.8% Sample 3 25269 29048 29153 26765 27558 1630.37 5.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1547 1291 1390 1463 1422 94.19 6.6% Sample 1 1547 1291 1390 1463 1422 94.19 6.6% Sample 2 3580 3482 3689 3752 3625 103.31 2.8% Sample 2 3580 3482 3689 3752 3625 103.31 2.8% Sample 3 25269 29048 29153 26765 27558 1630.37 5.9% Sample 3 25269 29048 29153 26765 27558 1630.37 5.9%

Synonyms

Pulmonary surfactant-associated protein D; PSP-D; SP-D; Collectin-7; Lung surfactant protein D; SFTPD; COLEC7, PSPD, SFTP4

Gene Name

SFTPD

Gene ID

6441

UniProt

P35247

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: A21-F375

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Expressed in lung, brain, pancreas and adipose tissue (mainly mature adipocytes) . .

Applications

ELISA

Field of Research

Calcium Binding Proteins, Calcium Signaling, Cancer, Cancer Metabolism, Cell Biology, Defensin, Human Protein, Immunology, Innate Immunity, Macrophage/Inflammation, Metabolism, Microbiology, Oxidative Stress, Pathways and Processes, Protein, Redox Metabolism, Signal Transduction, Signaling Pathway

Standard

40 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

625 pg/mL - 40,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1547 3580 25269 1591 3588 23717 Standard deviation 92.82 221.96 1693.02 111.37 279.86 1683.9 CV (%) 6% 6.2% 6.7% 7% 7.8% 7.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1547 3580 25269 1591 3588 23717 Standard deviation 92.82 221.96 1693.02 111.37 279.86 1683.9 CV (%) 6% 6.2% 6.7% 7% 7.8% 7.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1547 3580 25269 1591 3588 23717 Mean (pg/ml) 1547 3580 25269 1591 3588 23717 Standard deviation 92.82 221.96 1693.02 111.37 279.86 1683.9 Standard deviation 92.82 221.96 1693.02 111.37 279.86 1683.9 CV (%) 6% 6.2% 6.7% 7% 7.8% 7.1% CV (%) 6% 6.2% 6.7% 7% 7.8% 7.1%

Sensitivity

<20 pg/mL

Function

Contributes to the lung's defense against inhaled microorganisms, organic antigens and toxins. Interacts with compounds such as bacterial lipopolysaccharides, oligosaccharides and fatty acids and modulates leukocyte action in immune response. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties. .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

37728 MW

Specificity

Natural and recombinant human SP-D

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Pulmonary surfactant-associated protein D

Subcellular Location

Secreted, extracellular space, extracellular matrix. Secreted, extracellular space, surface film.

Sequence Similarities

Belongs to the SFTPD family.

Protein Name

Pulmonary surfactant-associated protein D

Sample Data

Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 O.D. 0.09 0.138 0.179 0.286 0.497 0.766 1.456 2.084 Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 O.D. 0.09 0.138 0.179 0.286 0.497 0.766 1.456 2.084 Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 O.D. 0.09 0.138 0.179 0.286 0.497 0.766 1.456 2.084 O.D. 0.09 0.138 0.179 0.286 0.497 0.766 1.456 2.084

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.