Human IL-13/Interleukin-13 ELISA Kit PicoKine®

Human IL-13 ELISA Kit PicoKine® (96 Tests) . Quantitate Human IL13 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Interleukin 13 (IL-13) is a protein that in humans is encoded by the IL13 gene. IL-13 is cytokine secreted by many cell types, but especially T helper type 2 (Th2) cells which is an important mediator of allergic inflammation and disease. IL-13 induces its effects through a multi-subunit receptor that includes the alpha chain of the IL-4 receptor (IL-4R alpha) and at least one of two known IL-13-specific binding chains. Most of the biological effects of IL-13, like those of IL-4, are linked to a single transcription factor, signal transducer and activator of transcription 6 (STAT6) . The IL13 gene is clustered with IL3, IL5, IL4, and CSF2 on 5q.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 34 30 29 33 31 2.06 6.6% Sample 2 190 180 201 184 188 7.91 4.2% Sample 3 438 424 460 467 447 17.16 3.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 34 30 29 33 31 2.06 6.6% Sample 1 34 30 29 33 31 2.06 6.6% Sample 2 190 180 201 184 188 7.91 4.2% Sample 2 190 180 201 184 188 7.91 4.2% Sample 3 438 424 460 467 447 17.16 3.8% Sample 3 438 424 460 467 447 17.16 3.8%

Synonyms

Interleukin-13; IL-13; IL13; NC30

Gene Name

IL13

Gene ID

3596

UniProt

P35225

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: G21-N132

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Cytokines, Immunology, Innate Immunity, Interleukins

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 34 190 438 32 176 440 Standard deviation 1.93 10.64 24.09 2.36 10.56 29.92 CV (%) 5.7% 5.6% 5.5% 7.4% 6% 6.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 34 190 438 32 176 440 Standard deviation 1.93 10.64 24.09 2.36 10.56 29.92 CV (%) 5.7% 5.6% 5.5% 7.4% 6% 6.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 34 190 438 32 176 440 Mean (pg/ml) 34 190 438 32 176 440 Standard deviation 1.93 10.64 24.09 2.36 10.56 29.92 Standard deviation 1.93 10.64 24.09 2.36 10.56 29.92 CV (%) 5.7% 5.6% 5.5% 7.4% 6% 6.8% CV (%) 5.7% 5.6% 5.5% 7.4% 6% 6.8%

Sensitivity

<10 pg/mL

Function

Cytokine. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

15816 MW

Specificity

Natural and recombinant human IL-13

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Interleukin-13

Subcellular Location

Secreted.

Sequence Similarities

Belongs to the IL-4/IL-13 family.

Protein Name

Interleukin-13

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.021 0.072 0.117 0.195 0.362 0.702 1.288 2.097 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.021 0.072 0.117 0.195 0.362 0.702 1.288 2.097 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.021 0.072 0.117 0.195 0.362 0.702 1.288 2.097 O.D. 0.021 0.072 0.117 0.195 0.362 0.702 1.288 2.097

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.