Human PDGF-BB ELISA Kit PicoKine®

Platelet-derived growth factor subunit B is a protein that in humans is encoded by the PDGFB gene. The protein encoded by this gene is a member of the platelet-derived growth factor family. This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. This gene is mapped to 22q13.1. Growth factor plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. This gene plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 94 89 99 93 93 3.56 3.8% Sample 2 199 217 232 218 216 11.71 5.4% Sample 3 788 792 795 811 796 8.73 1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 94 89 99 93 93 3.56 3.8% Sample 1 94 89 99 93 93 3.56 3.8% Sample 2 199 217 232 218 216 11.71 5.4% Sample 2 199 217 232 218 216 11.71 5.4% Sample 3 788 792 795 811 796 8.73 1% Sample 3 788 792 795 811 796 8.73 1%

Platelet-derived growth factor subunit B; PDGF subunit B; PDGF-2; Platelet-derived growth factor B chain; Platelet-derived growth factor beta polypeptide; Proto-oncogene c-Sis; INN: Becaplermin; PDGFB; PDGF2; SIS

Expressed at high levels in the heart, brain (sustantia nigra), placenta and fetal kidney. Expressed at moderate levels in the brain (hippocampus), skeletal muscle, kidney and lung.

Atherosclerosis, Cancer, Cancer Metabolism, Cardiovascular, Growth Factors, Growth Factors/Hormones, Immunology, Metabolism, Metabolism Processes, Neurology Process, Neuroscience, Pathways and Processes, Platelets, Response To Hypoxia, Secreted Molecules, Serum Proteins, Signal Transduction, Vascular Inflammation

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 94 199 788 88 194 725 Standard deviation 4.13 12.53 39.4 5.09 12.80 36.25 CV (%) 4.4% 6.6% 5% 5.9% 6.6% 5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 94 199 788 88 194 725 Standard deviation 4.13 12.53 39.4 5.09 12.80 36.25 CV (%) 4.4% 6.6% 5% 5.9% 6.6% 5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 94 199 788 88 194 725 Mean (pg/ml) 94 199 788 88 194 725 Standard deviation 4.13 12.53 39.4 5.09 12.80 36.25 Standard deviation 4.13 12.53 39.4 5.09 12.80 36.25 CV (%) 4.4% 6.6% 5% 5.9% 6.6% 5% CV (%) 4.4% 6.6% 5% 5.9% 6.6% 5%

Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin (PubMed:26599395) . Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA (By similarity) .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.011 0.071 0.133 0.247 0.468 0.866 1.581 2.505 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.011 0.071 0.133 0.247 0.468 0.866 1.581 2.505 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.011 0.071 0.133 0.247 0.468 0.866 1.581 2.505 O.D. 0.011 0.071 0.133 0.247 0.468 0.866 1.581 2.505

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.