Mouse SDF-1/CXCL12 ELISA Kit PicoKine®

SDF-1 (stromal cell-derived factor-1) is small cytokine belonging to the chemokine family that is officially designated Chemokine (C-X-C motif) ligand 12 (CXCL12) . This gene is located on chromosome 10q11.1. SDF-1 is produced in two forms, SDF-1alpha/CXCL12a and SDF-1beta/CXCL12b, by alternate splicing of the same gene. Chemokines are characterized by the presence of four conserved cysteines, which form two disulfide bonds. The CXCL12 proteins belong to the group of CXC chemokines, whose initial pair of cysteines are separated by one intervening amino acid. CXCL12 is strongly chemotactic for lymphocytes. CXCL12 was shown to be expressed in many tissues in mice (including brain, thymus, heart, lung, liver, kidney, spleen and bone marrow) . CXCL12 is a highly efficacious lymphocyte chemoattractant. In addition, CXCL12 induces intracellular actin polymerization in lymphocytes. CXCL12 is a substrate for the matrix metalloproteinase-2, which cleaves an CXCL12 N-terminal tetrapeptide. The standard product used in this kit is recombinant SDF-1 with the molecular mass of 8Kda.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 118 122 115 109 116 4.18 3.6% Sample 2 692 705 712 699 702 42.82 6.1% Sample 3 1469 1455 1545 1547 1504 82.72 5.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 118 122 115 109 116 4.18 3.6% Sample 1 118 122 115 109 116 4.18 3.6% Sample 2 692 705 712 699 702 42.82 6.1% Sample 2 692 705 712 699 702 42.82 6.1% Sample 3 1469 1455 1545 1547 1504 82.72 5.5% Sample 3 1469 1455 1545 1547 1504 82.72 5.5%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 118 692 1469 115 707 1562 Standard deviation 5.31 49.13 60.23 5.64 36.76 90.6 CV (%) 4.5% 7.1% 4.1% 4.9% 5.2% 5.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 118 692 1469 115 707 1562 Standard deviation 5.31 49.13 60.23 5.64 36.76 90.6 CV (%) 4.5% 7.1% 4.1% 4.9% 5.2% 5.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 118 692 1469 115 707 1562 Mean (pg/ml) 118 692 1469 115 707 1562 Standard deviation 5.31 49.13 60.23 5.64 36.76 90.6 Standard deviation 5.31 49.13 60.23 5.64 36.76 90.6 CV (%) 4.5% 7.1% 4.1% 4.9% 5.2% 5.8% CV (%) 4.5% 7.1% 4.1% 4.9% 5.2% 5.8%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.066 0.131 0.173 0.254 0.438 0.748 1.369 2.013 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.066 0.131 0.173 0.254 0.438 0.748 1.369 2.013 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.066 0.131 0.173 0.254 0.438 0.748 1.369 2.013 O.D. 0.066 0.131 0.173 0.254 0.438 0.748 1.369 2.013

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.