Rat Neuropilin-2 ELISA Kit PicoKine®

Rat Neuropilin-2 ELISA Kit PicoKine® (96 Tests) . Quantitate Rat Nrp2 in cell culture supernatants and cell lysates. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

NRP2 (Neuropilin-2), also called Npn2 or VEGF165R2, encodes a member of the neuropilin family of receptor proteins. The soluble NRP2 was secreted as a 62.5-kD protein following transfection in Chinese hamster ovary cells. The NRP2 gene is mapped to 2q33.3. The NRP2 gene contains 17 exons and spans about 112 kb. This protein may play a role in cardiovascular development, axon guidance, and tumorigenesis. Mice with null mutations in genes encoding Sema3F, and its holoreceptor components Npn2 and plexin A3 (PLEXA3), exhibit increased dentate gyrus granule cell and cortical layer V pyramidal neuron spine number and size, and also aberrant spine distribution. Moreover, Sema3F promotes loss of spines and excitatory synapses in dissociated neurons in vitro, and in NRP2-null brain slices cortical layer V and dentate gyrus granule cells exhibit increased miniature excitatory postsynaptic current frequency. These disparate effects of secreted semaphorins are reflected in the restricted dendritic localization of NRP2 to apical dendrites and of Npn1 to all dendrites of cortical pyramidal neurons.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 312 302 337 325 319 13.2 4.1% Sample 2 1117 1021 1010 950 1024 59.85 5.8% Sample 3 5486 5356 5497 5236 5393 106.63 1.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 312 302 337 325 319 13.2 4.1% Sample 1 312 302 337 325 319 13.2 4.1% Sample 2 1117 1021 1010 950 1024 59.85 5.8% Sample 2 1117 1021 1010 950 1024 59.85 5.8% Sample 3 5486 5356 5497 5236 5393 106.63 1.9% Sample 3 5486 5356 5497 5236 5393 106.63 1.9%

Synonyms

Neuropilin-2; Vascular endothelial cell growth factor 165 receptor 2; Nrp2

Gene Name

NRP2

Gene ID

81527

UniProt

O35276

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q23-D857

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Found in certain neuronal populations of the CNS and in other non-neuronal tissues including mesenchymal tissue lining in the ribs.

Applications

ELISA

Field of Research

Axonal Guidance Proteins, Cell Type Marker, Growth and Development, Neurology Process, Neuron Marker, Neuroscience

Standard

10 ng/Tube

Sample Type

Cell culture supernatants and cell lysates.

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 312 1117 5486 297 1022 5862 Standard deviation 16.53 44.68 274.3 21.38 48.03 310.68 CV (%) 5.3% 4% 5% 7.2% 4.7% 5.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 312 1117 5486 297 1022 5862 Standard deviation 16.53 44.68 274.3 21.38 48.03 310.68 CV (%) 5.3% 4% 5% 7.2% 4.7% 5.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 312 1117 5486 297 1022 5862 Mean (pg/ml) 312 1117 5486 297 1022 5862 Standard deviation 16.53 44.68 274.3 21.38 48.03 310.68 Standard deviation 16.53 44.68 274.3 21.38 48.03 310.68 CV (%) 5.3% 4% 5% 7.2% 4.7% 5.3% CV (%) 5.3% 4% 5% 7.2% 4.7% 5.3%

Sensitivity

<10 pg/mL

Function

High affinity receptor for semaphorins 3C, 3F, VEGF-165 and VEGF-145 isoforms of VEGF, and the PLGF-2 isoform of PGF.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

103897 MW

Specificity

Natural and recombinant rat Neuropilin-2

Gene Name Synonym

Neuropilin-2

Subcellular Location

Membrane; Single-pass type I membrane protein.

Sequence Similarities

Belongs to the neuropilin family.

Protein Name

Neuropilin-2

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.019 0.06 0.092 0.178 0.406 0.799 1.611 2.283 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.019 0.06 0.092 0.178 0.406 0.799 1.611 2.283 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.019 0.06 0.092 0.178 0.406 0.799 1.611 2.283 O.D. 0.019 0.06 0.092 0.178 0.406 0.799 1.611 2.283

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.