Mouse Neuropilin-2 ELISA Kit PicoKine®

Mouse Neuropilin-2 ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Nrp2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

NRP2 (Neuropilin-2), also called Npn2 or VEGF165R2, encodes a member of the neuropilin family of receptor proteins. The soluble NRP2 was secreted as a 62.5-kD protein following transfection in Chinese hamster ovary cells. The NRP2 gene is mapped to 2q33.3. The NRP2 gene contains 17 exons and spans about 112 kb. This protein may play a role in cardiovascular development, axon guidance, and tumorigenesis. Mice with null mutations in genes encoding Sema3F, and its holoreceptor components Npn2 and plexin A3 (PLEXA3), exhibit increased dentate gyrus granule cell and cortical layer V pyramidal neuron spine number and size, and also aberrant spine distribution. Moreover, Sema3F promotes loss of spines and excitatory synapses in dissociated neurons in vitro, and in NRP2-null brain slices cortical layer V and dentate gyrus granule cells exhibit increased miniature excitatory postsynaptic current frequency. These disparate effects of secreted semaphorins are reflected in the restricted dendritic localization of NRP2 to apical dendrites and of Npn1 to all dendrites of cortical pyramidal neurons.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 267 276 277 301 280 12.59 4.4% Sample 2 1352 1396 1389 1292 1357 41.21 3% Sample 3 5179 5231 5457 4899 5191 198.59 3.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 267 276 277 301 280 12.59 4.4% Sample 1 267 276 277 301 280 12.59 4.4% Sample 2 1352 1396 1389 1292 1357 41.21 3% Sample 2 1352 1396 1389 1292 1357 41.21 3% Sample 3 5179 5231 5457 4899 5191 198.59 3.8% Sample 3 5179 5231 5457 4899 5191 198.59 3.8%

Synonyms

Neuropilin-2; Vascular endothelial cell growth factor 165 receptor 2; Nrp2

Gene Name

NRP2

Gene ID

18187

UniProt

O35375

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q23-D863

Capture Antibody

Monoclonal antibody from rat

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Expressed in developing CNS, PNS and in some nonneural tissues including limb buds, developing bones, muscles, intestinal epithelium, kidney, lung and submandibular gland.

Applications

ELISA

Field of Research

Axonal Guidance Proteins, Cell Type Marker, Growth and Development, Neurology Process, Neuron Marker, Neuroscience

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 267 1352 5179 243 1366 4747 Standard deviation 17.62 66.24 346.99 16.52 77.86 394 CV (%) 6.6% 4.9% 6.7% 6.8% 5.7% 8.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 267 1352 5179 243 1366 4747 Standard deviation 17.62 66.24 346.99 16.52 77.86 394 CV (%) 6.6% 4.9% 6.7% 6.8% 5.7% 8.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 267 1352 5179 243 1366 4747 Mean (pg/ml) 267 1352 5179 243 1366 4747 Standard deviation 17.62 66.24 346.99 16.52 77.86 394 Standard deviation 17.62 66.24 346.99 16.52 77.86 394 CV (%) 6.6% 4.9% 6.7% 6.8% 5.7% 8.3% CV (%) 6.6% 4.9% 6.7% 6.8% 5.7% 8.3%

Sensitivity

<10 pg/mL

Function

High affinity receptor for semaphorins 3C, 3F, VEGF-165 and VEGF-145 isoforms of VEGF, and the PLGF-2 isoform of PGF.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

104631 MW

Specificity

Natural and recombinant mouse Neuropilin-2

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Neuropilin-2

Subcellular Location

Membrane; Single-pass type I membrane protein.

Sequence Similarities

Belongs to the neuropilin family.

Protein Name

Neuropilin-2

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.052 0.183 0.142 0.234 0.432 0.723 1.216 1.931 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.052 0.183 0.142 0.234 0.432 0.723 1.216 1.931 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.052 0.183 0.142 0.234 0.432 0.723 1.216 1.931 O.D. 0.052 0.183 0.142 0.234 0.432 0.723 1.216 1.931

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.