Human ADAM9 ELISA Kit PicoKine®

Human ADAM9 ELISA Kit PicoKine® (96 Tests) . Quantitate Human ADAM9 in cell culture supernatants, serum and plasma (heparin) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Disintegrin and metalloproteinase domain-containing protein 9, also known as MCMP or MDC9 is an enzyme that in humans is encoded by the ADAM9 gene. This gene encodes a member of the ADAM ('a disintegrin and metalloproteinase') family and exhibits a number of characteristics of class III reprolysins. This gene is mapped to 8p11.22. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 38 41 35 42 39 2.73 7% Sample 2 260 225 245 259 247 14.14 5.7% Sample 3 407 428 485 443 440 28.56 6.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 38 41 35 42 39 2.73 7% Sample 1 38 41 35 42 39 2.73 7% Sample 2 260 225 245 259 247 14.14 5.7% Sample 2 260 225 245 259 247 14.14 5.7% Sample 3 407 428 485 443 440 28.56 6.4% Sample 3 407 428 485 443 440 28.56 6.4%

Synonyms

Disintegrin and metalloproteinase domain-containing protein 9; ADAM 9;3.4.24.-; Cellular disintegrin-related protein; Meltrin-gamma; Metalloprotease/disintegrin/cysteine-rich protein 9; Myeloma cell metalloproteinase; ADAM9; KIAA0021, MCMP, MDC9, MLTNG

Gene Name

ADAM9

Gene ID

8754

UniProt

Q13443

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: A29-D697

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Widely expressed. Expressed in chondrocytes. Isoform 2 is highly expressed in liver and heart. .

Applications

ELISA

Field of Research

Signal Transduction

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin) .

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 38 260 407 38 269 375 Standard deviation 2.35 16% 17.9 2.43 19.09 20.62 CV (%) 6.2% 6% 4.4% 6.4% 7.1% 5.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 38 260 407 38 269 375 Standard deviation 2.35 16% 17.9 2.43 19.09 20.62 CV (%) 6.2% 6% 4.4% 6.4% 7.1% 5.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 38 260 407 38 269 375 Mean (pg/ml) 38 260 407 38 269 375 Standard deviation 2.35 16% 17.9 2.43 19.09 20.62 Standard deviation 2.35 16% 17.9 2.43 19.09 20.62 CV (%) 6.2% 6% 4.4% 6.4% 7.1% 5.5% CV (%) 6.2% 6% 4.4% 6.4% 7.1% 5.5%

Sensitivity

<10 pg/mL

Function

Probable zinc protease. May mediate cell-cell or cell- matrix interactions. Isoform 2 displays alpha-secretase activity for APP. .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

90556 MW

Specificity

Natural and recombinant human ADAM9

Anticoagulant

Heparin

Gene Name Synonym

Disintegrin and metalloproteinase domain-containing protein 9

Subcellular Location

Isoform 1: Cell membrane; Single-pass type I membrane protein.

Sequence Similarities

Contains 1 disintegrin domain.

Protein Name

Disintegrin and metalloproteinase domain-containing protein 9

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.068 0.107 0.171 0.262 0.442 0.728 1.3 2.254 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.068 0.107 0.171 0.262 0.442 0.728 1.3 2.254 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.068 0.107 0.171 0.262 0.442 0.728 1.3 2.254 O.D. 0.068 0.107 0.171 0.262 0.442 0.728 1.3 2.254

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.