Human ADAMTS1 ELISA Kit PicoKine®

ADAMTS1 (A Disintegrin-Like and Metalloproteinase with Thrombospondin Type 1 Motif, 1), also known as METH1, is an enzyme that in humans is encoded by the ADAMTS1 gene. ADAMTS is a family of proteins believed to be anchored to the extracellular matrix (ECM) through interactions with aggregan or other matrix components by one or more thrombospondin type 1 motifs. ADAMTS1 gene is mapped to 21q21.2 based on sequence similarity between the ADAMTS1 sequence and a chromosome 21q21.2 clone. It has been found that ADAMTS1 can disrupt angiogenesis in vivo and in vitro more efficiently than ADAMTS8, THBS1, or endostatin. What’s more, ADAMTS1 disrupts angiogenesis in vivo and in vitro more efficiently than ADAMTS8, THBS1, or endostatin.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 145 144 155 149 148 4.32 2.9% Sample 2 681 610 663 663 654 26.58 4% Sample 3 2886 2884 2690 2607 2766 121.83 4.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 145 144 155 149 148 4.32 2.9% Sample 1 145 144 155 149 148 4.32 2.9% Sample 2 681 610 663 663 654 26.58 4% Sample 2 681 610 663 663 654 26.58 4% Sample 3 2886 2884 2690 2607 2766 121.83 4.4% Sample 3 2886 2884 2690 2607 2766 121.83 4.4%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 145 681 2886 132 732 2843 Standard deviation 8.84 37.45 207.79 10.56 51.97 213.22 CV (%) 6.1% 5.5% 7.2% 8% 7.1% 7.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 145 681 2886 132 732 2843 Standard deviation 8.84 37.45 207.79 10.56 51.97 213.22 CV (%) 6.1% 5.5% 7.2% 8% 7.1% 7.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 145 681 2886 132 732 2843 Mean (pg/ml) 145 681 2886 132 732 2843 Standard deviation 8.84 37.45 207.79 10.56 51.97 213.22 Standard deviation 8.84 37.45 207.79 10.56 51.97 213.22 CV (%) 6.1% 5.5% 7.2% 8% 7.1% 7.5% CV (%) 6.1% 5.5% 7.2% 8% 7.1% 7.5%

Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover (By similarity) . Has angiogenic inhibitor activity. Active metalloprotease, which may be associated with various inflammatory processes as well as development of cancer cachexia. May play a critical role in follicular rupture. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.048 0.107 0.151 0.229 0.42 0.729 1.366 2.138 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.048 0.107 0.151 0.229 0.42 0.729 1.366 2.138 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.048 0.107 0.151 0.229 0.42 0.729 1.366 2.138 O.D. 0.048 0.107 0.151 0.229 0.42 0.729 1.366 2.138

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.