Human SDC1 / Syndecan-1 / CD138 ELISA Kit PicoKine®

Human SDC1 / Syndecan-1 / CD138 ELISA Kit PicoKine® (96 Tests) . Quantitate Human SDC1 in cell culture supernatants and serum. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Syndecan 1, also known as SYND1 or CD138, is a protein which in humans is encoded by the SDC1 gene. The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. This gene is mapped to 2p24.1. The syndecans mediate cell binding, cell signaling, cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan 1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. What’s more, Syndecan-1 is also a sponge for growth factors, with binding largely via heparan sulfate chains.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 632 571 608 604 603 21.72 3.6% Sample 2 2595 2506 2397 2686 2546 107 4.2% Sample 3 8396 8079 9304 8737 8629 453.89 5.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 632 571 608 604 603 21.72 3.6% Sample 1 632 571 608 604 603 21.72 3.6% Sample 2 2595 2506 2397 2686 2546 107 4.2% Sample 2 2595 2506 2397 2686 2546 107 4.2% Sample 3 8396 8079 9304 8737 8629 453.89 5.2% Sample 3 8396 8079 9304 8737 8629 453.89 5.2%

Synonyms

Syndecan-1; SYND1; CD138; SDC1; SDC

Gene Name

SDC1

Gene ID

6382

UniProt

P18827

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q23-G254

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Cancer, Cancer Metabolism, Cell Adhesion Proteins, Cell Type Marker, Cytoskeleton/ECM, ECM Proteins, Extracellular Matrix, Hematopoietic Progenitors, Invasion/Microenvironment, Lipid and Lipoprotein Metabolism, Lipid Metabolism, Lymphoid, Membrane Proteins, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Metabolism of Lipids and Lipoproteins, Neurogenesis, Neurology Process, Neuron Marker, Neuroscience, Pathways and Processes, Signal Transduction, Stem Cells, Synapse Marker

Standard

20 ng/Tube

Sample Type

Cell culture supernatants and serum.

Detection Range

312 pg/mL - 20,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 632 25676 8396 676 2617 8785 Standard deviation 26.51 171.27 545.74 31.09 196.27 588.59 CV (%) 4.1% 6.6% 6.5% 4.6% 7.5% 6.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 632 25676 8396 676 2617 8785 Standard deviation 26.51 171.27 545.74 31.09 196.27 588.59 CV (%) 4.1% 6.6% 6.5% 4.6% 7.5% 6.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 632 25676 8396 676 2617 8785 Mean (pg/ml) 632 25676 8396 676 2617 8785 Standard deviation 26.51 171.27 545.74 31.09 196.27 588.59 Standard deviation 26.51 171.27 545.74 31.09 196.27 588.59 CV (%) 4.1% 6.6% 6.5% 4.6% 7.5% 6.7% CV (%) 4.1% 6.6% 6.5% 4.6% 7.5% 6.7%

Sensitivity

<10 pg/mL

Function

Cell surface proteoglycan that bears both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

32462 MW

Specificity

Natural and recombinant human Syndecan-1

Gene Name Synonym

Syndecan-1

Subcellular Location

Membrane; Single-pass type I membrane protein. Secreted. Shedding of the ectodomain produces a soluble form.

Sequence Similarities

Belongs to the syndecan proteoglycan family.

Protein Name

Syndecan-1

Sample Data

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.011 0.085 0.184 0.263 0.523 0.944 1.558 2.173 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.011 0.085 0.184 0.263 0.523 0.944 1.558 2.173 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.011 0.085 0.184 0.263 0.523 0.944 1.558 2.173 O.D. 0.011 0.085 0.184 0.263 0.523 0.944 1.558 2.173

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.