Human Mesothelin ELISA Kit PicoKine®

Human Mesothelin ELISA Kit PicoKine® (96 Tests) . Quantitate Human MSLN in cell culture supernatants, serum, plasma (heparin, EDTA), saliva and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Mesothelin, also known as MSLN or MPF is a protein that in humans is encoded by the MSLN gene. This gene is mapped to 16p13.3. This gene encodes a precursor protein that is cleaved into two products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiation factor functions as a cytokine that can stimulate colony formation in bone marrow megakaryocytes. Mesothelian is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 307 282 307 344 310 22.12 7.1% Sample 2 1077 1082 1223 1218 1150 70.54 6.1% Sample 3 6075 5852 5500 5776 5800 205.48 3.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 307 282 307 344 310 22.12 7.1% Sample 1 307 282 307 344 310 22.12 7.1% Sample 2 1077 1082 1223 1218 1150 70.54 6.1% Sample 2 1077 1082 1223 1218 1150 70.54 6.1% Sample 3 6075 5852 5500 5776 5800 205.48 3.5% Sample 3 6075 5852 5500 5776 5800 205.48 3.5%

Synonyms

Mesothelin; CAK1 antigen; Pre-pro-megakaryocyte-potentiating factor; Megakaryocyte-potentiating factor; MPF; Mesothelin, cleaved form; MSLN; MPF

Gene Name

MSLN

Gene ID

10232

UniProt

Q13421

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: E296-G580

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Expressed in lung. Expressed at low levels in heart, placenta and kidney. Expressed in mesothelial cells. Highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level) . .

Applications

ELISA

Field of Research

Cancer, Cell Type Markers, Tags & Cell Markers, Tumor Antigens, Tumor Associated, Tumor Biomarkers, Tumor Immunology, Tumor-Associated Antigens

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA), saliva and urine.

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 307 1077 6075 320 1138 6301 Standard deviation 12.58 52.77 443.47 17.28 70.55 516.68 CV (%) 4.1% 4.9% 7.3% 5.4% 6.2% 8.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 307 1077 6075 320 1138 6301 Standard deviation 12.58 52.77 443.47 17.28 70.55 516.68 CV (%) 4.1% 4.9% 7.3% 5.4% 6.2% 8.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 307 1077 6075 320 1138 6301 Mean (pg/ml) 307 1077 6075 320 1138 6301 Standard deviation 12.58 52.77 443.47 17.28 70.55 516.68 Standard deviation 12.58 52.77 443.47 17.28 70.55 516.68 CV (%) 4.1% 4.9% 7.3% 5.4% 6.2% 8.2% CV (%) 4.1% 4.9% 7.3% 5.4% 6.2% 8.2%

Sensitivity

<10 pg/mL

Function

Membrane-anchored forms may play a role in cellular adhesion.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

68986 MW

Specificity

Natural and recombinant human Mesothelin

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Mesothelin

Subcellular Location

Cell membrane; Lipid-anchor, GPI-anchor. Golgi apparatus.

Protein Name

Mesothelin

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.051 0.151 0.199 0.331 0.548 0.932 1.622 2.269 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.051 0.151 0.199 0.331 0.548 0.932 1.622 2.269 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.051 0.151 0.199 0.331 0.548 0.932 1.622 2.269 O.D. 0.051 0.151 0.199 0.331 0.548 0.932 1.622 2.269

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.