Mouse TIMP-4 ELISA Kit PicoKine®

Metalloproteinase inhibitor 4 is an enzyme that in humans is encoded by the TIMP4 gene. This gene belongs to the tissue inhibitor of metalloproteinases gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. TIMP4 gene is mapped to chromosome 3p25. TIMP4 gene contains 5 exons that span 6 kb of genomic DNA. The gene is expressed as a 1.4-kb transcript abundant in heart and present at low levels in several other tissues. Expressed TIMP4 inhibits MMPs in vitro. What’s more, TIMP-4 binds both progelatinase A and the C domain in a similar manner to that of TIMP-2.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 131 113 132 117 123 8.37 6.8% Sample 2 690 674 647 709 680 22.72 3.3% Sample 3 2000 2212 1975 2086 2068 92.64 4.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 131 113 132 117 123 8.37 6.8% Sample 1 131 113 132 117 123 8.37 6.8% Sample 2 690 674 647 709 680 22.72 3.3% Sample 2 690 674 647 709 680 22.72 3.3% Sample 3 2000 2212 1975 2086 2068 92.64 4.4% Sample 3 2000 2212 1975 2086 2068 92.64 4.4%

Angiogenesis, Cancer, Cardiovascular, Cell Biology, ECM Enzymes, Extracellular Matrix, Invasion/Microenvironment, Protease Inhibitors, Proteolysis/Ubiquitin

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 131 690 2000 137 710 2055 Standard deviation 6.94 44.85 156 8.63 51.83 199.33 CV (%) 5.3% 6.5% 7.8% 7.3%% 7.3% 9.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 131 690 2000 137 710 2055 Standard deviation 6.94 44.85 156 8.63 51.83 199.33 CV (%) 5.3% 6.5% 7.8% 7.3%% 7.3% 9.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 131 690 2000 137 710 2055 Mean (pg/ml) 131 690 2000 137 710 2055 Standard deviation 6.94 44.85 156 8.63 51.83 199.33 Standard deviation 6.94 44.85 156 8.63 51.83 199.33 CV (%) 5.3% 6.5% 7.8% 7.3%% 7.3% 9.7% CV (%) 5.3% 6.5% 7.8% 7.3%% 7.3% 9.7%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.014 0.085 0.144 0.282 0.507 0.946 1.568 2.147 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.014 0.085 0.144 0.282 0.507 0.946 1.568 2.147 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.014 0.085 0.144 0.282 0.507 0.946 1.568 2.147 O.D. 0.014 0.085 0.144 0.282 0.507 0.946 1.568 2.147

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.