Mouse TIMP-4 ELISA Kit PicoKine®

Mouse TIMP-4 ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Timp4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Metalloproteinase inhibitor 4 is an enzyme that in humans is encoded by the TIMP4 gene. This gene belongs to the tissue inhibitor of metalloproteinases gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. TIMP4 gene is mapped to chromosome 3p25. TIMP4 gene contains 5 exons that span 6 kb of genomic DNA. The gene is expressed as a 1.4-kb transcript abundant in heart and present at low levels in several other tissues. Expressed TIMP4 inhibits MMPs in vitro. What’s more, TIMP-4 binds both progelatinase A and the C domain in a similar manner to that of TIMP-2.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 131 113 132 117 123 8.37 6.8% Sample 2 690 674 647 709 680 22.72 3.3% Sample 3 2000 2212 1975 2086 2068 92.64 4.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 131 113 132 117 123 8.37 6.8% Sample 1 131 113 132 117 123 8.37 6.8% Sample 2 690 674 647 709 680 22.72 3.3% Sample 2 690 674 647 709 680 22.72 3.3% Sample 3 2000 2212 1975 2086 2068 92.64 4.4% Sample 3 2000 2212 1975 2086 2068 92.64 4.4%

Synonyms

Metalloproteinase inhibitor 4; Tissue inhibitor of metalloproteinases 4; TIMP-4; Timp4

Gene Name

TIMP4

Gene ID

110595

UniProt

Q9JHB3

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: C30-P224

Capture Antibody

Monoclonal antibody from rat

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Expressed in brain, heart, ovary and skeletal muscle.

Applications

ELISA

Field of Research

Angiogenesis, Cancer, Cardiovascular, Cell Biology, ECM Enzymes, Extracellular Matrix, Invasion/Microenvironment, Protease Inhibitors, Proteolysis/Ubiquitin

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

78 pg/mL - 5,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 131 690 2000 137 710 2055 Standard deviation 6.94 44.85 156 8.63 51.83 199.33 CV (%) 5.3% 6.5% 7.8% 7.3%% 7.3% 9.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 131 690 2000 137 710 2055 Standard deviation 6.94 44.85 156 8.63 51.83 199.33 CV (%) 5.3% 6.5% 7.8% 7.3%% 7.3% 9.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 131 690 2000 137 710 2055 Mean (pg/ml) 131 690 2000 137 710 2055 Standard deviation 6.94 44.85 156 8.63 51.83 199.33 Standard deviation 6.94 44.85 156 8.63 51.83 199.33 CV (%) 5.3% 6.5% 7.8% 7.3%% 7.3% 9.7% CV (%) 5.3% 6.5% 7.8% 7.3%% 7.3% 9.7%

Sensitivity

<10 pg/mL

Function

Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

25774 MW

Specificity

Natural and recombinant mouse TIMP-4

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Metalloproteinase inhibitor 4

Subcellular Location

Secreted.

Sequence Similarities

Belongs to the protease inhibitor I35 (TIMP) family.

Protein Name

Metalloproteinase inhibitor 4

Sample Data

Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.014 0.085 0.144 0.282 0.507 0.946 1.568 2.147 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.014 0.085 0.144 0.282 0.507 0.946 1.568 2.147 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.014 0.085 0.144 0.282 0.507 0.946 1.568 2.147 O.D. 0.014 0.085 0.144 0.282 0.507 0.946 1.568 2.147

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.