Mouse MADCAM-1 ELISA Kit PicoKine®

MADCAM1 (Mucosal Vascular Addressin Cell Adhesion Molecule 1), also known as MACAM1, is a protein that is encoded by the MADCAM1 gene. By PCR-based analysis of somatic cell hybrids, Leung et al (1997) mapped the MACAM1 gene to chromosome 19. The protein encoded by this gene is an endothelil cell adhesion molecule that interacts preferentially with the leukocyte beta7 integrin LPAM-1 (alpha4 / beta7), L-selectin, and VLA-4 (alpha4/beta1) on myeloid cells to leukocytes into mucosal and inflamed tissues. It is a member of the immunoglobulin superfamily and is similar to ICAM-1 and VCAM-1.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 187 202 201 193 195 6.13 3.1% Sample 2 1248 1232 1145 1158 1195 44.84 3.7% Sample 3 5083 5932 5979 5400 5598 374.54 6.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 187 202 201 193 195 6.13 3.1% Sample 1 187 202 201 193 195 6.13 3.1% Sample 2 1248 1232 1145 1158 1195 44.84 3.7% Sample 2 1248 1232 1145 1158 1195 44.84 3.7% Sample 3 5083 5932 5979 5400 5598 374.54 6.6% Sample 3 5083 5932 5979 5400 5598 374.54 6.6%

Highly expressed on high endothelial venules (HEV) of organized intestinal lymphoid tissues like the Peyer patches and mesenteric lymph nodes, and in the lamina propria of the intestine. Some expression found in the spleen, and low levels of expression in the peripheral lymph nodes and the lactating mammary gland. No expression was detected in the liver, kidneys, lungs or in normal brain. Expressed as well in brain endothelioma cells, and mucosal tissues which are in a chronic state of inflammation, such as inflammed pancreas. .

Angiogenesis, Cardiovascular, Cell Adhesion, Cell Adhesion Proteins, Cytoskeleton/ECM, Immunology, Innate Immunity, Integrins, Macrophage/Inflammation, Neuroscience, Receptors, Signal Transduction

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 187 1248 5083 189 1354 5007 Standard deviation 13.09 59.9 376.14 15.3 75.82 380.53 CV (%) 7% 4.8% 7.4% 8.1% 5.6% 7.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 187 1248 5083 189 1354 5007 Standard deviation 13.09 59.9 376.14 15.3 75.82 380.53 CV (%) 7% 4.8% 7.4% 8.1% 5.6% 7.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 187 1248 5083 189 1354 5007 Mean (pg/ml) 187 1248 5083 189 1354 5007 Standard deviation 13.09 59.9 376.14 15.3 75.82 380.53 Standard deviation 13.09 59.9 376.14 15.3 75.82 380.53 CV (%) 7% 4.8% 7.4% 8.1% 5.6% 7.6% CV (%) 7% 4.8% 7.4% 8.1% 5.6% 7.6%

Cell adhesion leukocyte receptor expressed by mucosal venules, helps to lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both the integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes. Both isoform 1 and isoform 2 can adhere to integrin alpha-4/beta-7. Isoform 2, lacking the mucin-like domain, may be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L-selectin binding. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.044 0.072 0.151 0.321 0.621 1.121 1.838 2.415 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.044 0.072 0.151 0.321 0.621 1.121 1.838 2.415 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.044 0.072 0.151 0.321 0.621 1.121 1.838 2.415 O.D. 0.044 0.072 0.151 0.321 0.621 1.121 1.838 2.415

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.