Mouse GPNMB/Osteoactivin ELISA Kit PicoKine®

Transmembrane glycoprotein NMB is a protein that is encoded by the GPNMB gene. In osteoblast progenitor cells, GPNMB works as a positive regulator of osteoblast differentiation during later stages of matrix maturation and mineralization that is mediated at least in part by BMP-2 in a SMAD1 dependent manner to promote osteoblast differentiation. GPNMB can enhance the repairing process in bone fracture, demonstrated by its high expression during chondrogenesis (soft callus) and osteogenesis (hard callus) compared to the intact femurs that is why Osteoactivin (OA) could be a novel therapeutic agent used to treat generalized osteoporosis or localized osteopenia during fracture repair by stimulating bone growth and regeneration. Similarly, GPNMB expression increases during osteoclast differentiation and it is functionally implicated in this process, possibly by promoting the fusion of osteoclast progenitor cells.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 265 290 285 290 282 10.3 3.6% Sample 2 1462 1602 1474 1607 1536 68.4 4.4% Sample 3 4620 4438 4374 4575 4501 99.66 2.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 265 290 285 290 282 10.3 3.6% Sample 1 265 290 285 290 282 10.3 3.6% Sample 2 1462 1602 1474 1607 1536 68.4 4.4% Sample 2 1462 1602 1474 1607 1536 68.4 4.4% Sample 3 4620 4438 4374 4575 4501 99.66 2.2% Sample 3 4620 4438 4374 4575 4501 99.66 2.2%

In early development, mRNA is detected at high levels in the outer layer of the retina. Later in development expression gets restricted to the retinal pigment epithelium and iris. Overexpressed in mammary cancer cells possessing a strong bone metastatic phenotype. .

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 265 1462 4620 283 1371 4849 Standard deviation 13.78 73.1 258.72 19.24 80.88 353.97 CV (%) 5.2% 5% 5.6% 6.8% 5.9% 7.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 265 1462 4620 283 1371 4849 Standard deviation 13.78 73.1 258.72 19.24 80.88 353.97 CV (%) 5.2% 5% 5.6% 6.8% 5.9% 7.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 265 1462 4620 283 1371 4849 Mean (pg/ml) 265 1462 4620 283 1371 4849 Standard deviation 13.78 73.1 258.72 19.24 80.88 353.97 Standard deviation 13.78 73.1 258.72 19.24 80.88 353.97 CV (%) 5.2% 5% 5.6% 6.8% 5.9% 7.3% CV (%) 5.2% 5% 5.6% 6.8% 5.9% 7.3%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cell membrane ; Single-pass type I membrane protein . Endomembrane system ; Single-pass type I membrane protein . Cytoplasmic vesicle membrane ; Single-pass type I membrane protein . Melanosome . Vesicular, endosomal like structures.

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.026 0.141 0.257 0.469 0.82 1.336 1.832 2.182 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.026 0.141 0.257 0.469 0.82 1.336 1.832 2.182 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.026 0.141 0.257 0.469 0.82 1.336 1.832 2.182 O.D. 0.026 0.141 0.257 0.469 0.82 1.336 1.832 2.182

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.