Mouse Chemerin/RARRES2 ELISA Kit PicoKine®

Chemerin, also known as RARRES2 or TIG2, is a protein that is encoded by the RARRES2 gene. It is mapped to 7q36.1. Chemerin is a potent chemoattractant specific for antigen-presenting cells that requires proteolytic activation and acts as a ligand for the G protein-coupled receptor CMKLR1 (also known as ChemR23) . It is a 14 kDa protein secreted in an inactive form as prochemerin and is activated through cleavage of the C-terminus by inflammatory and coagulation serine proteases. Chemerin was found to stimulate chemotaxis of dendritic cells and macrophages to the site of inflammation. What’s more, the active protein has several roles, including that as an adipokine, and is truncated on both termini from the proprotein.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1599 1433 1534 1458 1506 65.31 4.3% Sample 2 7530 6996 6760 7509 7198 331.5 4.6% Sample 3 22585 25134 23932 25295 24236 1089.27 4.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1599 1433 1534 1458 1506 65.31 4.3% Sample 1 1599 1433 1534 1458 1506 65.31 4.3% Sample 2 7530 6996 6760 7509 7198 331.5 4.6% Sample 2 7530 6996 6760 7509 7198 331.5 4.6% Sample 3 22585 25134 23932 25295 24236 1089.27 4.4% Sample 3 22585 25134 23932 25295 24236 1089.27 4.4%

Expressed in the differentiated adipocytes (at protein level) . Abundantly expressed in the liver, adipose tissue including visceral, epididymal, and brown adipose tissue. .

Cancer, Cardiovascular, Lipid and Lipoprotein Metabolism, Lipid Metabolism, Lipids/Lipoproteins, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Signal Transduction

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1599 7530 22585 1551 7093 23589 Standard deviation 100.73 444.27 1558.36 97.71 524.88 1957.88 CV (%) 7.4%% 6.9%% 6.9% 7.4%% 7.4% 8.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1599 7530 22585 1551 7093 23589 Standard deviation 100.73 444.27 1558.36 97.71 524.88 1957.88 CV (%) 7.4%% 6.9%% 6.9% 7.4%% 7.4% 8.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1599 7530 22585 1551 7093 23589 Mean (pg/ml) 1599 7530 22585 1551 7093 23589 Standard deviation 100.73 444.27 1558.36 97.71 524.88 1957.88 Standard deviation 100.73 444.27 1558.36 97.71 524.88 1957.88 CV (%) 7.4%% 6.9%% 6.9% 7.4%% 7.4% 8.3% CV (%) 7.4%% 6.9%% 6.9% 7.4%% 7.4% 8.3%

Adipocyte-secreted protein (adipokine) that regulates adipogenesis, metabolism and inflammation through activation of the chemokine-like receptor 1 (CMKLR1) . Its other ligands include G protein-coupled receptor 1 (GPR1) and chemokine receptor-like 2 (CCRL2) . Positively regulates adipocyte differentiation, modulates the expression of adipocyte genes involved in lipid and glucose metabolism and might play a role in angiogenesis, a process essential for the expansion of white adipose tissue. Also acts as a proinflammatory adipokine, causing an increase in secretion of proinflammatory and prodiabetic adipokines, which further impair adipose tissue metabolic function and have negative systemic effects including impaired insulin sensitivity, altered glucose and lipid metabolism, and a decrease in vascular function in other tissues. Can have both pro- and anti-inflammatory properties depending on the modality of enzymatic cleavage by different classes of proteases. Acts as a chemotactic factor for leukocyte populations expressing CMKLR1, particularly immature plasmacytoid dendritic cells, but also immature myeloid DCs, macrophages and natural killer cells. Exerts an anti-inflammatory role by preventing TNF/TNFA-induced VCAM1 expression and monocytes adhesion in vascular endothelial cells. The effect is mediated via inhibiting activation of NF-kappa-B and CRK/p38 through stimulation of AKT1/NOS3 signaling and nitric oxide production. Exhibits an antimicrobial function in the skin. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.042 0.109 0.17 0.305 0.558 0.915 1.749 2.004 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.042 0.109 0.17 0.305 0.558 0.915 1.749 2.004 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.042 0.109 0.17 0.305 0.558 0.915 1.749 2.004 O.D. 0.042 0.109 0.17 0.305 0.558 0.915 1.749 2.004

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.