Rat IL-3 ELISA Kit PicoKine®

The gene IL-3 encodes interleukin 3, a hematopoietic colony-stimulating factor (CSF) that is capable of supporting the proliferation of a broad range of hematopoietic cell types. Interleukin-3 (IL-3), a protein of 140 amino acids, is chemically synthesized by means of an automated peptide synthesizer and is shown to have the biological activities attributed to native IL-3. The cDNA sequence for murine interleukin-3, one of the colony stimulating factors that regulate haematopoiesis, codes for a polypeptide of 166 amino acids including a putative signal peptide. The mouse IL 3 gene is located on chromosome 11. The human gene encoding IL 3 is tandemly arrayed on the long arm of chromosome 5.5 The standard product used in this kit is recombinant human IL-3, consisting of 133 amino acids with the molecular mass of 15KDa.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 16 15 15 15 15 0.43 2.8% Sample 2 91 90 85 99 91 5.01 5.5% Sample 3 256 243 230 237 241 9.55 3.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 16 15 15 15 15 0.43 2.8% Sample 1 16 15 15 15 15 0.43 2.8% Sample 2 91 90 85 99 91 5.01 5.5% Sample 2 91 90 85 99 91 5.01 5.5% Sample 3 256 243 230 237 241 9.55 3.9% Sample 3 256 243 230 237 241 9.55 3.9%

Interleukin-3; IL-3; Hematopoietic growth factor; Mast cell growth factor; MCGF; Multipotential colony-stimulating factor; P-cell-stimulating factor; Il3; Il-3

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 16 91 256 14 84 249 Standard deviation 0.8 5.36 16.64 0.77 5.37 17.43 CV (%) 5% 6.5%% 6.5% 5.5% 6.4% 7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 16 91 256 14 84 249 Standard deviation 0.8 5.36 16.64 0.77 5.37 17.43 CV (%) 5% 6.5%% 6.5% 5.5% 6.4% 7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 16 91 256 14 84 249 Mean (pg/ml) 16 91 256 14 84 249 Standard deviation 0.8 5.36 16.64 0.77 5.37 17.43 Standard deviation 0.8 5.36 16.64 0.77 5.37 17.43 CV (%) 5% 6.5%% 6.5% 5.5% 6.4% 7% CV (%) 5% 6.5%% 6.5% 5.5% 6.4% 7%

Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.029 0.138 0.213 0.365 0.65 1.074 1.673 2.331 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.029 0.138 0.213 0.365 0.65 1.074 1.673 2.331 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.029 0.138 0.213 0.365 0.65 1.074 1.673 2.331 O.D. 0.029 0.138 0.213 0.365 0.65 1.074 1.673 2.331

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.