Human DKK-3 ELISA Kit PicoKine®

Dickkopf-related protein 3 is a protein that in humans is encoded by the DKK3 gene. This gene encodes a protein that is a member of the dickkopf family. It is mapped to 11p15.3. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. The expression of this gene is decreased in a variety of cancer cell lines and it may function as a tumor suppressor gene. Members of the Dkk-related family display unique patterns of mRNA expression in human and mouse tissues, and are secreted when expressed in 293T cells. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 308 334 342 343 331 14.14 4.2% Sample 2 1957 1955 1972 1710 1898 109.02 5.7% Sample 3 5464 5826 5731 5903 5731 165.75 2.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 308 334 342 343 331 14.14 4.2% Sample 1 308 334 342 343 331 14.14 4.2% Sample 2 1957 1955 1972 1710 1898 109.02 5.7% Sample 2 1957 1955 1972 1710 1898 109.02 5.7% Sample 3 5464 5826 5731 5903 5731 165.75 2.8% Sample 3 5464 5826 5731 5903 5731 165.75 2.8%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 308 1957 5464 336 2016 5345 Standard deviation 17.24 133.07 262.27 26.80 139.1 272.59 CV (%) 6.8% 6.8% 4.8% 7.5% 6.9% 5.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 308 1957 5464 336 2016 5345 Standard deviation 17.24 133.07 262.27 26.80 139.1 272.59 CV (%) 6.8% 6.8% 4.8% 7.5% 6.9% 5.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 308 1957 5464 336 2016 5345 Mean (pg/ml) 308 1957 5464 336 2016 5345 Standard deviation 17.24 133.07 262.27 26.80 139.1 272.59 Standard deviation 17.24 133.07 262.27 26.80 139.1 272.59 CV (%) 6.8% 6.8% 4.8% 7.5% 6.9% 5.1% CV (%) 6.8% 6.8% 4.8% 7.5% 6.9% 5.1%

Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero- posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease (By similarity) . .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.024 0.096 0.167 0.288 0.527 0.961 1.526 2.035 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.024 0.096 0.167 0.288 0.527 0.961 1.526 2.035 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.024 0.096 0.167 0.288 0.527 0.961 1.526 2.035 O.D. 0.024 0.096 0.167 0.288 0.527 0.961 1.526 2.035

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.