Human CD93/C1qR ELISA Kit PicoKine®

CD93 (Cluster of Differentiation 93) is a protein that in humans is encoded by the CD93 gene. CD93 belongs to the Group XIV C-Type lectin family and is mapped to 20p11.21. The protein encoded by this gene is a cell-surface glycoprotein and type I membrane protein that was originally identified as a myeloid cell-specific marker. The encoded protein was once thought to be a receptor for C1q, but now is thought to instead be involved in intercellular adhesion and in the clearance of apoptotic cells. The intracellular cytoplasmic tail of this protein has been found to interact with moesin, a protein known to play a role in linking transmembrane proteins to the cytoskeleton and in the remodelling of the cytoskeleton. It has also showed that this receptor is a positive marker for all bone marrow-repopulating stem cells because it is expressed on both CD34-negative and CD34-positive stem cells from umbilical cord blood and adult bone marrow. In addition, highly purified lineage-negative cells with the CD93 protein not only have bone marrow-repopulating capacity but also can differentiate into human hepatocytes in vivo.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 94 96 95 92 94 1.47 1.5% Sample 2 706 763 758 718 736 24.68 3.3% Sample 3 2947 2642 2748 2847 2796 113.38 4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 94 96 95 92 94 1.47 1.5% Sample 1 94 96 95 92 94 1.47 1.5% Sample 2 706 763 758 718 736 24.68 3.3% Sample 2 706 763 758 718 736 24.68 3.3% Sample 3 2947 2642 2748 2847 2796 113.38 4% Sample 3 2947 2642 2748 2847 2796 113.38 4%

Complement component C1q receptor; C1q/MBL/SPA receptor; C1qR; C1qR (p) ; C1qRp; CDw93; Complement component 1 q subcomponent receptor 1; Matrix-remodeling-associated protein 4; CD93; CD93; C1QR1, MXRA4

Highly expressed in endothelial cells, platelets, cells of myeloid origin, such as monocytes and neutrophils. Not expressed in cells of lymphoid origin.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 94 706 2947 99 657 2665 Standard deviation 7.23 53.65 197.44 8.31 58.47 191.88 CV (%) 7.7% 7.6% 6.7% 8.3% 8.9% 7.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 94 706 2947 99 657 2665 Standard deviation 7.23 53.65 197.44 8.31 58.47 191.88 CV (%) 7.7% 7.6% 6.7% 8.3% 8.9% 7.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 94 706 2947 99 657 2665 Mean (pg/ml) 94 706 2947 99 657 2665 Standard deviation 7.23 53.65 197.44 8.31 58.47 191.88 Standard deviation 7.23 53.65 197.44 8.31 58.47 191.88 CV (%) 7.7% 7.6% 6.7% 8.3% 8.9% 7.2% CV (%) 7.7% 7.6% 6.7% 8.3% 8.9% 7.2%

Receptor (or element of a larger receptor complex) for C1q, mannose-binding lectin (MBL2) and pulmonary surfactant protein A (SPA) . May mediate the enhancement of phagocytosis in monocytes and macrophages upon interaction with soluble defense collagens. May play a role in intercellular adhesion.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.005 0.06 0.133 0.273 0.548 1.004 1.488 1.99 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.005 0.06 0.133 0.273 0.548 1.004 1.488 1.99 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.005 0.06 0.133 0.273 0.548 1.004 1.488 1.99 O.D. 0.005 0.06 0.133 0.273 0.548 1.004 1.488 1.99

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.