Human SPARCL1/SPARC-like protein 1 ELISA Kit PicoKine®

SPARCL1 (SPARC-Like Protein 1), also known as HEVIN, is a protein that in humans is encoded by the SPARCL1 gene. The cells in high endothelial venules (HEVs) in lymphoid tissues have a plump, almost cuboidal, appearance and support high levels of lymphocyte extravasation from blood, possibly due to the presence of desmosome-like junctions rather than tight junctions in the HEVs. In chronic inflammation, the activated endothelium of nonlymphoid tissues acquires an HEV-like morphology and function. SPARCL1 is highly expressed in HEV and is thought to contribute to the induction or maintenance of features of the HEV endothelium that facilitate lymphocyte migration.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 81 81 84 89 83 3.26 3.9% Sample 2 322 287 303 283 298 15.36 5.1% Sample 3 1261 1280 1392 1332 1316 50.87 3.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 81 81 84 89 83 3.26 3.9% Sample 1 81 81 84 89 83 3.26 3.9% Sample 2 322 287 303 283 298 15.36 5.1% Sample 2 322 287 303 283 298 15.36 5.1% Sample 3 1261 1280 1392 1332 1316 50.87 3.8% Sample 3 1261 1280 1392 1332 1316 50.87 3.8%

Highly expressed in lymph node, brain, heart, lung, skeletal muscle, ovary, small intestine, and colon, with lower levels in placenta, pancreas, testis, spleen, and thymus, and no expression in kidney, liver, and peripheral blood leukocytes.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 81 322 1261 78 330 1329 Standard deviation 4.45 17.71 79.44 4.75 22.11 94.35 CV (%) 5.5% 5.5% 6.7% 6.1% 6.7% 7.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 81 322 1261 78 330 1329 Standard deviation 4.45 17.71 79.44 4.75 22.11 94.35 CV (%) 5.5% 5.5% 6.7% 6.1% 6.7% 7.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 81 322 1261 78 330 1329 Mean (pg/ml) 81 322 1261 78 330 1329 Standard deviation 4.45 17.71 79.44 4.75 22.11 94.35 Standard deviation 4.45 17.71 79.44 4.75 22.11 94.35 CV (%) 5.5% 5.5% 6.7% 6.1% 6.7% 7.1% CV (%) 5.5% 5.5% 6.7% 6.1% 6.7% 7.1%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.003 0.035 0.062 0.113 0.205 0.458 0.828 1.843 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.003 0.035 0.062 0.113 0.205 0.458 0.828 1.843 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.003 0.035 0.062 0.113 0.205 0.458 0.828 1.843 O.D. 0.003 0.035 0.062 0.113 0.205 0.458 0.828 1.843

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.