Mouse TREM-1 ELISA Kit PicoKine®

Trem1, Triggering receptor expressed on myeloid cells-1, is encoded by Trem1 gene. The expression of Trem1 is in monocytes and neutrophils but not in lymphocytes, dendritic cells, or other cell types. Trem1 is a 30-kD glycoprotein that is reduced to 26 kD by deglycosylation, in agreement with the predicted molecular mass. The Trem1 gene which contains 4 exons maps to chromosome 6p21.1, within a TREM gene cluster and the mouse Trem1 gene maps to chromosome 17 in a region that shows homology of synteny to human chromosome 6. The expression of Trem1 is upregulated by stimulation with lipopolysaccharide (LPS), gram-negative bacteria, and fungi. Cross-linking of Trem1 on neutrophils induces interleukin-8 (IL8) and myeloperoxidase secretion, while cross-linking on monocytes induces not only secretion of IL8 but also of monocyte chemotactic protein-1 (MCP1, or SCYA2) and tumor necrosis factor (TNF) ; MCP1 and TNF secretion could be further upregulated by LPS-mediated priming. Trem1 engagement also induces upregulation of adhesion molecules (e.g., ITGB1) and costimulatory molecules (e.g., CD40) . Trem1 is associated with DAP12 (TYROBP), a molecule frequently associated with activating receptors.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 104 89 102 94 97 6.05 6.2% Sample 2 219 224 232 232 226 5.53 2.4% Sample 3 892 782 872 830 844 42.21 5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 104 89 102 94 97 6.05 6.2% Sample 1 104 89 102 94 97 6.05 6.2% Sample 2 219 224 232 232 226 5.53 2.4% Sample 2 219 224 232 232 226 5.53 2.4% Sample 3 892 782 872 830 844 42.21 5% Sample 3 892 782 872 830 844 42.21 5%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 104 219 892 113 227 835 Standard deviation 7.28 12.26 40.14 9.26 13.84 53.44 CV (%) 7% 5.6% 4.5% 8.2% 6.1% 6.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 104 219 892 113 227 835 Standard deviation 7.28 12.26 40.14 9.26 13.84 53.44 CV (%) 7% 5.6% 4.5% 8.2% 6.1% 6.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 104 219 892 113 227 835 Mean (pg/ml) 104 219 892 113 227 835 Standard deviation 7.28 12.26 40.14 9.26 13.84 53.44 Standard deviation 7.28 12.26 40.14 9.26 13.84 53.44 CV (%) 7% 5.6% 4.5% 8.2% 6.1% 6.4% CV (%) 7% 5.6% 4.5% 8.2% 6.1% 6.4%

Stimulates neutrophil and monocyte-mediated inflammatory responses. Triggers release of pro-inflammatory chemokines and cytokines, as well as increased surface expression of cell activation markers. Amplifier of inflammatory responses that are triggered by bacterial and fungal infections and is a crucial mediator of septic shock (By similarity) . .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.032 0.086 0.126 0.216 0.401 0.755 1.37 2.384 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.032 0.086 0.126 0.216 0.401 0.755 1.37 2.384 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.032 0.086 0.126 0.216 0.401 0.755 1.37 2.384 O.D. 0.032 0.086 0.126 0.216 0.401 0.755 1.37 2.384

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.