Mouse Amphiregulin (AR) ELISA Kit PicoKine®

Amphiregulin, also known as AREG, is a protein that is encoded by the AREG gene. The protein encoded by this gene is a member of the epidermal growth factor family. This gene is mapped to mouse chromosome 5. It is an autocrine growth factor as well as a mitogen forastrocytes, Schwann cells, fibroblasts. Amphiregulin is related to toepidermal growth factor (EGF) and transforming growth factor alpha (TGF-alpha) . This protein interacts with the epidermal growth factor receptor to promote the growth of normal epithelial cells. It has been found that transgene integration and subsequent expression of Amphiregulin in basal keratinocytes correlated with a psoriasis-like skin phenotype, and its expression was increased in parallel with typical Th2 cytokines. Amphiregulin is also an important paracrine mediator of estrogen function specifically required for puberty-induced ductal elongation but not for any earlier or later developmental stages, and it can enhance resistance to nematodes.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 31 30 32 32 31 0.82 2.6% Sample 2 206 224 213 202 211 8.34 3.9% Sample 3 409 443 416 466 433 22.65 5.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 31 30 32 32 31 0.82 2.6% Sample 1 31 30 32 32 31 0.82 2.6% Sample 2 206 224 213 202 211 8.34 3.9% Sample 2 206 224 213 202 211 8.34 3.9% Sample 3 409 443 416 466 433 22.65 5.2% Sample 3 409 443 416 466 433 22.65 5.2%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 31 206 409 33 191 397 Standard deviation 2.01 9.27 19.22 2.14 12.22 26.2 CV (%) 6.5% 4.5% 4.7% 6.5% 6.4% 6.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 31 206 409 33 191 397 Standard deviation 2.01 9.27 19.22 2.14 12.22 26.2 CV (%) 6.5% 4.5% 4.7% 6.5% 6.4% 6.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 31 206 409 33 191 397 Mean (pg/ml) 31 206 409 33 191 397 Standard deviation 2.01 9.27 19.22 2.14 12.22 26.2 Standard deviation 2.01 9.27 19.22 2.14 12.22 26.2 CV (%) 6.5% 4.5% 4.7% 6.5% 6.4% 6.6% CV (%) 6.5% 4.5% 4.7% 6.5% 6.4% 6.6%

Ligand of the EGF receptor/EGFR. Autocrine growth factor as well as a mitogen for a broad range of target cells including astrocytes, Schwann cells and fibroblasts.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.08 0.146 0.214 0.326 0.496 0.836 1.293 1.947 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.08 0.146 0.214 0.326 0.496 0.836 1.293 1.947 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.08 0.146 0.214 0.326 0.496 0.836 1.293 1.947 O.D. 0.08 0.146 0.214 0.326 0.496 0.836 1.293 1.947

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.