Mouse VEGFR3/FLT4 ELISA Kit PicoKine®

Fms-related tyrosine kinase 4, also known as FLT4 or VEGFR3, is a protein which in humans is encoded by the FLT4 gene. It is mapped to 5q35.3. This gene encodes a tyrosine kinase receptor for vascular endothelial growth factors C and D. The protein is thought to be involved in lymphangiogenesis and maintenance of the lymphatic endothelium. FLT4 has an essential role in the development of the embryonic cardiovascular system before the emergence of the lymphatic vessels. It has been found that FLT4, which provides proangiogenic signaling when expressed on endothelium, may also have antiangiogenic properties when expressed at an avascular site by nonendothelial cells. FLT4 is also regarded as a regulator of vascular network formation.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 287 282 308 309 296 12.13 4% Sample 2 1392 1474 1350 1411 1406 44.66 3.1% Sample 3 5844 5515 6505 5591 5863 389.74 6.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 287 282 308 309 296 12.13 4% Sample 1 287 282 308 309 296 12.13 4% Sample 2 1392 1474 1350 1411 1406 44.66 3.1% Sample 2 1392 1474 1350 1411 1406 44.66 3.1% Sample 3 5844 5515 6505 5591 5863 389.74 6.6% Sample 3 5844 5515 6505 5591 5863 389.74 6.6%

Vascular endothelial growth factor receptor 3; VEGFR-3;2.7.10.1; Fms-like tyrosine kinase 4; FLT-4; Tyrosine-protein kinase receptor FLT4; Flt4; Flt-4, Vegfr3

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 287 1392 5844 306 1363 5406 Standard deviation 22.38 87.69 344.79 29.07 99.49 389.23 CV (%) 7.8% 7.3%% 5.9% 9.5% 7.3% 7.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 287 1392 5844 306 1363 5406 Standard deviation 22.38 87.69 344.79 29.07 99.49 389.23 CV (%) 7.8% 7.3%% 5.9% 9.5% 7.3% 7.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 287 1392 5844 306 1363 5406 Mean (pg/ml) 287 1392 5844 306 1363 5406 Standard deviation 22.38 87.69 344.79 29.07 99.49 389.23 Standard deviation 22.38 87.69 344.79 29.07 99.49 389.23 CV (%) 7.8% 7.3%% 5.9% 9.5% 7.3% 7.2% CV (%) 7.8% 7.3%% 5.9% 9.5% 7.3% 7.2%

Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFC and VEGFD, and plays an essential role in adult lymphangiogenesis and in the development of the vascular network and the cardiovascular system during embryonic development. Promotes proliferation, survival and migration of endothelial cells, and regulates angiogenic sprouting. Signaling by activated FLT4 leads to enhanced production of VEGFC, and to a lesser degree VEGFA, thereby creating a positive feedback loop that enhances FLT4 signaling. Modulates KDR signaling by forming heterodimers. Mediates activation of the MAPK1/ERK2, MAPK3/ERK1 signaling pathway, of MAPK8 and the JUN signaling pathway, and of the AKT1 signaling pathway. Phosphorylates SHC1. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3- kinase. Promotes phosphorylation of MAPK8 at 'Thr-183' and 'Tyr- 185', and of AKT1 at 'Ser-473'. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.058 0.118 0.179 0.3 0.557 0.962 1.638 2.243 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.058 0.118 0.179 0.3 0.557 0.962 1.638 2.243 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.058 0.118 0.179 0.3 0.557 0.962 1.638 2.243 O.D. 0.058 0.118 0.179 0.3 0.557 0.962 1.638 2.243

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.