Mouse ANGPTL3 ELISA Kit PicoKine®

ANGPTL3, also known as Angiopoietin-like 3 or HYPLl, is a protein that in humans is encoded by the ANGPTL3 gene. It is mapped to 1p31.3. The protein encoded by this gene is a member of the angiopoietin-like family of secreted factors. It is expressed predominantly in the liver, and has the characteristic structure of angiopoietins, consisting of a signal peptide, N-terminal coiled-coil domain, and the C-terminal fibrinogen (FBN) -like domain. ANGPTL3 also acts as dual inhibitor of lipoprotein lipase (LPL) and endothelial lipase (EL), and increases plasma triglyceride and HDL cholesterol in rodents. It can inhibit endothelial lipase to catalyze HDL-phospholipid and increase HDL-PL levels.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 234 260 243 235 243 10.41 4.2% Sample 2 1410 1366 1348 1447 1392 38.59 2.7% Sample 3 5833 5711 5318 5602 5616 190.46 3.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 234 260 243 235 243 10.41 4.2% Sample 1 234 260 243 235 243 10.41 4.2% Sample 2 1410 1366 1348 1447 1392 38.59 2.7% Sample 2 1410 1366 1348 1447 1392 38.59 2.7% Sample 3 5833 5711 5318 5602 5616 190.46 3.3% Sample 3 5833 5711 5318 5602 5616 190.46 3.3%

Angiogenesis, Cancer, Cardiovascular, Energy Metabolism, Energy Transfer Pathways, Growth Factors, Lipid Metabolism, Metabolic Signaling Pathways, Metabolism, Obesity, Pathways and Processes, Signal Transduction

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 234 1410 5833 215 1499 6259 Standard deviation 14.7%1 59.22 274.15 17.2 79.44 337.98 CV (%) 6.8% 4.2% 4.7% 8% 5.3% 5.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 234 1410 5833 215 1499 6259 Standard deviation 14.7%1 59.22 274.15 17.2 79.44 337.98 CV (%) 6.8% 4.2% 4.7% 8% 5.3% 5.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 234 1410 5833 215 1499 6259 Mean (pg/ml) 234 1410 5833 215 1499 6259 Standard deviation 14.7%1 59.22 274.15 17.2 79.44 337.98 Standard deviation 14.7%1 59.22 274.15 17.2 79.44 337.98 CV (%) 6.8% 4.2% 4.7% 8% 5.3% 5.4% CV (%) 6.8% 4.2% 4.7% 8% 5.3% 5.4%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.032 0.175 0.294 0.511 0.851 1.32 1.825 2.213 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.032 0.175 0.294 0.511 0.851 1.32 1.825 2.213 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.032 0.175 0.294 0.511 0.851 1.32 1.825 2.213 O.D. 0.032 0.175 0.294 0.511 0.851 1.32 1.825 2.213

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.