Mouse IL-20/Interleukin-20 ELISA Kit PicoKine®

IL-20, also known as Interleukin 20 or Zcyto10, is a protein that in humans is encoded by the IL20 gene. This gene is mapped to 1q32.1. IL-20 is a protein belonging to the IL-10 family of cytokines. This cytokine has been shown to transduce its signal through signal transducer and activator of transcription 3 (STAT3) in keratinocytes. A specific receptor for this cytokine is found to be expressed in skin and upregulated dramatically in psoriatic skin, suggesting a role for this protein in epidermal function and psoriasis. IL-20 regulates proliferation and differentiation of keratinocytes during inflammation, particularly inflammation associated with the skin. In addition, IL-20 also causes cell expansion of multipotential hematopoietic progenitor cells.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 93 89 90 83 88 3.63 4.1% Sample 2 232 246 237 225 235 7.64 3.2% Sample 3 997 874 895 860 906 53.71 5.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 93 89 90 83 88 3.63 4.1% Sample 1 93 89 90 83 88 3.63 4.1% Sample 2 232 246 237 225 235 7.64 3.2% Sample 2 232 246 237 225 235 7.64 3.2% Sample 3 997 874 895 860 906 53.71 5.9% Sample 3 997 874 895 860 906 53.71 5.9%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 93 232 997 88 234 940 Standard deviation 6.41 11.6 66.79 6.86 14.5 68.62 CV (%) 6.9% 5% 6.7% 7.8% 6.2% 7.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 93 232 997 88 234 940 Standard deviation 6.41 11.6 66.79 6.86 14.5 68.62 CV (%) 6.9% 5% 6.7% 7.8% 6.2% 7.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 93 232 997 88 234 940 Mean (pg/ml) 93 232 997 88 234 940 Standard deviation 6.41 11.6 66.79 6.86 14.5 68.62 Standard deviation 6.41 11.6 66.79 6.86 14.5 68.62 CV (%) 6.9% 5% 6.7% 7.8% 6.2% 7.3% CV (%) 6.9% 5% 6.7% 7.8% 6.2% 7.3%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.01 0.039 0.069 0.137 0.276 0.612 1.222 2.319 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.01 0.039 0.069 0.137 0.276 0.612 1.222 2.319 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.01 0.039 0.069 0.137 0.276 0.612 1.222 2.319 O.D. 0.01 0.039 0.069 0.137 0.276 0.612 1.222 2.319

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.