Mouse IL-20/Interleukin-20 ELISA Kit PicoKine®

Mouse IL-20 ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Il20 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

IL-20, also known as Interleukin 20 or Zcyto10, is a protein that in humans is encoded by the IL20 gene. This gene is mapped to 1q32.1. IL-20 is a protein belonging to the IL-10 family of cytokines. This cytokine has been shown to transduce its signal through signal transducer and activator of transcription 3 (STAT3) in keratinocytes. A specific receptor for this cytokine is found to be expressed in skin and upregulated dramatically in psoriatic skin, suggesting a role for this protein in epidermal function and psoriasis. IL-20 regulates proliferation and differentiation of keratinocytes during inflammation, particularly inflammation associated with the skin. In addition, IL-20 also causes cell expansion of multipotential hematopoietic progenitor cells.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 93 89 90 83 88 3.63 4.1% Sample 2 232 246 237 225 235 7.64 3.2% Sample 3 997 874 895 860 906 53.71 5.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 93 89 90 83 88 3.63 4.1% Sample 1 93 89 90 83 88 3.63 4.1% Sample 2 232 246 237 225 235 7.64 3.2% Sample 2 232 246 237 225 235 7.64 3.2% Sample 3 997 874 895 860 906 53.71 5.9% Sample 3 997 874 895 860 906 53.71 5.9%

Synonyms

Interleukin-20; IL-20; Cytokine Zcyto10; Il20; Zcyto10

Gene Name

IL20

Gene ID

58181

UniProt

Q9JKV9

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: L25-E176

Capture Antibody

Monoclonal antibody from rat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Cell Type Markers, Cytokines, Immunology, Innate Immunity, Interleukins

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 93 232 997 88 234 940 Standard deviation 6.41 11.6 66.79 6.86 14.5 68.62 CV (%) 6.9% 5% 6.7% 7.8% 6.2% 7.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 93 232 997 88 234 940 Standard deviation 6.41 11.6 66.79 6.86 14.5 68.62 CV (%) 6.9% 5% 6.7% 7.8% 6.2% 7.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 93 232 997 88 234 940 Mean (pg/ml) 93 232 997 88 234 940 Standard deviation 6.41 11.6 66.79 6.86 14.5 68.62 Standard deviation 6.41 11.6 66.79 6.86 14.5 68.62 CV (%) 6.9% 5% 6.7% 7.8% 6.2% 7.3% CV (%) 6.9% 5% 6.7% 7.8% 6.2% 7.3%

Sensitivity

<10 pg/mL

Function

Cytokine that may be involved in epidermal function and psoriasis. Acts through STAT3.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

20098 MW

Specificity

Natural and recombinant mouse IL-20

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Interleukin-20

Subcellular Location

Secreted.

Protein Name

Interleukin-20

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.01 0.039 0.069 0.137 0.276 0.612 1.222 2.319 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.01 0.039 0.069 0.137 0.276 0.612 1.222 2.319 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.01 0.039 0.069 0.137 0.276 0.612 1.222 2.319 O.D. 0.01 0.039 0.069 0.137 0.276 0.612 1.222 2.319

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.