Human JAM-A / F11R / Junctional Adhesion Molecule 1 ELISA Kit PicoKine®

Human JAM-A / F11R / Junctional Adhesion Molecule 1 ELISA Kit PicoKine® (96 Tests) . Quantitate Human F11R in cell culture supernatants and serum. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Junctional adhesion molecule A (JAM-A) is a protein that in humans is encoded by the F11R gene. It is mapped to 1q23.3. This gene is an immunoglobulin-like molecule that colocalizes with tight junctions in endothelium and epithelium and is also found on blood leukocytes and platelets. JAM-A plays an important role in the regulation of tight junction assembly in epithelia. In addition, it can act as a receptor for reovirus, a ligand for the integrin LFA1, involved in leukocyte transmigration, and a platelet receptor. JAM-A has a nonredundant role in controlling DC motility, trafficking to lymph nodes, and activation of specific immunity.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 81 88 85 93 86 4.38 5% Sample 2 325 306 319 326 319 7.96 2.4% Sample 3 1280 1301 1409 1335 1331 48.99 3.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 81 88 85 93 86 4.38 5% Sample 1 81 88 85 93 86 4.38 5% Sample 2 325 306 319 326 319 7.96 2.4% Sample 2 325 306 319 326 319 7.96 2.4% Sample 3 1280 1301 1409 1335 1331 48.99 3.6% Sample 3 1280 1301 1409 1335 1331 48.99 3.6%

Synonyms

Junctional adhesion molecule A; JAM-A; Junctional adhesion molecule 1; JAM-1; Platelet F11 receptor; Platelet adhesion molecule 1; PAM-1; CD321; F11R; JAM1, JCAM; UNQ264/PRO301

Gene Name

F11R

Gene ID

50848

UniProt

Q9Y624

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: S28-V238

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Atherosclerosis, Cardiovascular, Cell Adhesion, Cytoskeleton/ECM, Platelets, Signal Transduction, Tight Junctions, Vascular Inflammation

Standard

10 ng/Tube

Sample Type

Cell culture supernatants and serum.

Detection Range

46.9 pg/mL - 3,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 81 325 1280 73 310 1209 Standard deviation 5.83 16.9 67.84 5.32 16.43 93.09 CV (%) 7.2% 5.2% 5.3% 7.3% 5.3% 7.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 81 325 1280 73 310 1209 Standard deviation 5.83 16.9 67.84 5.32 16.43 93.09 CV (%) 7.2% 5.2% 5.3% 7.3% 5.3% 7.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 81 325 1280 73 310 1209 Mean (pg/ml) 81 325 1280 73 310 1209 Standard deviation 5.83 16.9 67.84 5.32 16.43 93.09 Standard deviation 5.83 16.9 67.84 5.32 16.43 93.09 CV (%) 7.2% 5.2% 5.3% 7.3% 5.3% 7.7% CV (%) 7.2% 5.2% 5.3% 7.3% 5.3% 7.7%

Sensitivity

<10 pg/mL

Function

Seems to play a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3. The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly (By similarity) . Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier. Involved in platelet activation. In case of orthoreovirus infection, serves as receptor for the virus. .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

32583 MW

Specificity

Natural and recombinant human JAM-A

Gene Name Synonym

Junctional adhesion molecule A

Subcellular Location

Cell junction, tight junction . Cell membrane ; Single-pass type I membrane protein . Localized at tight junctions of both epithelial and endothelial cells.

Sequence Similarities

Belongs to the immunoglobulin superfamily.

Protein Name

Junctional adhesion molecule A

Sample Data

Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.008 0.108 0.203 0.377 0.672 1.187 1.84 2.443 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.008 0.108 0.203 0.377 0.672 1.187 1.84 2.443 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.008 0.108 0.203 0.377 0.672 1.187 1.84 2.443 O.D. 0.008 0.108 0.203 0.377 0.672 1.187 1.84 2.443

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.