Human Livin ELISA Kit PicoKine®

BIRC7, also called KIAP or Livin, is a protein that in humans is encoded by the BIRC7 gene. This gene is mapped to 20q13.33. It encodes a member of the inhibitor of apoptosis protein (IAP) family, and contains a single copy of a baculovirus IAP repeat (BIR) as well as a RING-type zinc finger domain. The BIR domain is essential for inhibitory activity and interacts with caspases, while the RING finger domain sometimes enhances antiapoptotic activity but does not inhibit apoptosis alone. Elevated levels of the encoded protein may be associated with cancer progression and play a role in chemotherapy sensitivity. BIRC7 blocked apoptosis induced by BAX induction of cytochrome C release from mitochondria, and it also can block apoptosis induced by chemotherapeutic agents.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 344 365 383 379 368 15.08 4.1% Sample 2 1869 1916 1940 1875 1900 98.8 5.2% Sample 3 6693 6845 7026 6968 6883 371.68 5.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 344 365 383 379 368 15.08 4.1% Sample 1 344 365 383 379 368 15.08 4.1% Sample 2 1869 1916 1940 1875 1900 98.8 5.2% Sample 2 1869 1916 1940 1875 1900 98.8 5.2% Sample 3 6693 6845 7026 6968 6883 371.68 5.4% Sample 3 6693 6845 7026 6968 6883 371.68 5.4%

Baculoviral IAP repeat-containing protein 7; Kidney inhibitor of apoptosis protein; KIAP; Livin; ML-IAP; RING finger protein 50; RING-type E3 ubiquitin transferase BIRC7; Baculoviral IAP repeat-containing protein 7 30kDa subunit; Truncated livin; p30-Livin; tLivin; BIRC7; KIAP; LIVIN; MLIAP; RNF50; UNQ5800; PRO19607; PRO21344

Isoform 1 and isoform 2 are expressed at very low levels or not detectable in most adult tissues. Detected in adult heart, placenta, lung, lymph node, spleen and ovary, and in several carcinoma cell lines. Isoform 2 is detected in fetal kidney, heart and spleen, and at lower levels in adult brain, skeletal muscle and peripheral blood leukocytes.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 344 1869 6693 362 1964 6956 Standard deviation 22.02 80.37 401.58 24.25 104.09 507.79 CV (%) 6.4% 4.3% 6% 6.7% 5.3% 7.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 344 1869 6693 362 1964 6956 Standard deviation 22.02 80.37 401.58 24.25 104.09 507.79 CV (%) 6.4% 4.3% 6% 6.7% 5.3% 7.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 344 1869 6693 362 1964 6956 Mean (pg/ml) 344 1869 6693 362 1964 6956 Standard deviation 22.02 80.37 401.58 24.25 104.09 507.79 Standard deviation 22.02 80.37 401.58 24.25 104.09 507.79 CV (%) 6.4% 4.3% 6% 6.7% 5.3% 7.3% CV (%) 6.4% 4.3% 6% 6.7% 5.3% 7.3%

Apoptotic regulator capable of exerting proapoptotic and anti-apoptotic activities and plays crucial roles in apoptosis, cell proliferation, and cell cycle control. Its anti-apoptotic activity is mediated through the inhibition of CASP3, CASP7 and CASP9, as well as by its E3 ubiquitin-protein ligase activity. As it is a weak caspase inhibitor, its anti-apoptotic activity is thought to be due to its ability to ubiquitinate DIABLO/SMAC targeting it for degradation thereby promoting cell survival. May contribute to caspase inhibition, by blocking the ability of DIABLO/SMAC to disrupt XIAP/BIRC4-caspase interactions. Protects against apoptosis induced by TNF or by chemical agents such as adriamycin, etoposide or staurosporine. Suppression of apoptosis is mediated by activation of MAPK8/JNK1, and possibly also of MAPK9/JNK2. This activation depends on TAB1 and NR2C2/TAK1. In vitro, inhibits CASP3 and proteolytic activation of pro-CASP9. Isoform 1 blocks staurosporine-induced apoptosis. Isoform 2 blocks etoposide-induced apoptosis. Isoform 2 protects against natural killer (NK) cell killing whereas isoform 1 augments killing.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.058 0.108 0.133 0.213 0.343 0.572 0.977 1.751 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.058 0.108 0.133 0.213 0.343 0.572 0.977 1.751 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.058 0.108 0.133 0.213 0.343 0.572 0.977 1.751 O.D. 0.058 0.108 0.133 0.213 0.343 0.572 0.977 1.751

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.