Mouse IL-17C ELISA Kit PicoKine®

Mouse IL-17C ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Il17c in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

IL17C, also known as CX2, is a protein that in humans is encoded by the IL17C gene. IL17C is mapped to 16q24.3. The protein encoded by this gene is a T cell-derived cytokine that shares the sequence similarity with IL17. This cytokine was reported to stimulate the release of tumor necrosis factor alpha and interleukin 1 beta from a monocytic cell line. The expression of this cytokine was found to be restricted to activated T cells. IL17C is an essential autocrine cytokine regulating innate epithelial immune responses. It also plays an important role in the pathogenesis of inflammatory arthritis.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 150 157 168 157 158 6.44 4% Sample 2 1098 1141 972 1172 1095 76.12 6.9% Sample 3 6281 5461 5326 5304 5593 401.73 7.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 150 157 168 157 158 6.44 4% Sample 1 150 157 168 157 158 6.44 4% Sample 2 1098 1141 972 1172 1095 76.12 6.9% Sample 2 1098 1141 972 1172 1095 76.12 6.9% Sample 3 6281 5461 5326 5304 5593 401.73 7.1% Sample 3 6281 5461 5326 5304 5593 401.73 7.1%

Synonyms

Interleukin-17C; Il-17c; Cytokine CX2; Il17c

Gene Name

IL17C

Gene ID

234836

UniProt

Q8K4C5

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: D17-Q194

Capture Antibody

Monoclonal antibody from rat

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Expressed by epithelial cells after bacterial challenge. Low expression, if any, in lymphocytes. .

Applications

ELISA

Field of Research

Adaptive Immunity, Autoimmune, Cytokines, Immune System Diseases, Immunology, Innate Immunity, Interleukins

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

125 pg/mL - 8,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 150 1098 6281 140 1153 5857 Standard deviation 11.85 68.07 414.54 13.58 85.32 447.63 CV (%) 7.9% 6.2% 6.6% 9.7% 7.4% 7.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 150 1098 6281 140 1153 5857 Standard deviation 11.85 68.07 414.54 13.58 85.32 447.63 CV (%) 7.9% 6.2% 6.6% 9.7% 7.4% 7.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 150 1098 6281 140 1153 5857 Mean (pg/ml) 150 1098 6281 140 1153 5857 Standard deviation 11.85 68.07 414.54 13.58 85.32 447.63 Standard deviation 11.85 68.07 414.54 13.58 85.32 447.63 CV (%) 7.9% 6.2% 6.6% 9.7% 7.4% 7.6% CV (%) 7.9% 6.2% 6.6% 9.7% 7.4% 7.6%

Sensitivity

<10 pg/mL

Function

Cytokine that plays a crucial role in innate immunity of the epithelium, including to intestinal bacterial pathogens, in an autocrine manner. Stimulates the production of antibacterial peptides and proinflammatory molecules for host defense by signaling through the NFKB and MAPK pathways. Acts synergically with IL22, TNF and IL1B in inducing antibacterial peptides. May have protective function by maintaining epithelial homeostasis after an inflammatory challenge, such as that caused in the intestine by dextran sulfate sodium in a colitis model. May also promote an inflammatory phenotype, such as skin in a psoriasis model. Enhanced IL17C/IL17RE signaling may also lead to greater susceptibility to autoimmune diseases, such as autoimmune encephalitis. .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

21592 MW

Specificity

Natural and recombinant mouse IL-17C

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Interleukin-17C

Subcellular Location

Secreted .

Sequence Similarities

Belongs to the IL-17 family.

Protein Name

Interleukin-17C

Sample Data

Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.033 0.115 0.192 0.325 0.608 1.07 1.77 2.417 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.033 0.115 0.192 0.325 0.608 1.07 1.77 2.417 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.033 0.115 0.192 0.325 0.608 1.07 1.77 2.417 O.D. 0.033 0.115 0.192 0.325 0.608 1.07 1.77 2.417

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.