Human IL-17RB ELISA Kit PicoKine®

Human IL-17RB ELISA Kit PicoKine® (96 Tests) . Quantitate Human IL17RB in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

IL17RB, also known as CRL4, is a protein that in humans is encoded by the IL17RB gene. It is located on 3p21.1. IL17RB is produced by activated T cells exhibiting proinflammatory activities. The protein encoded by this gene is a cytokine receptor. This receptor specifically binds to IL17B and IL17E (IL25), but does not bind to IL17 (A) or IL17C. This receptor has been shown to mediate the activation of NF-kappaB and the production of IL8 induced by IL17E. And it may play a role in controlling the growth and/or differentiation of hematopoietic cells.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 303 331 321 324 319 10.32 3.2% Sample 2 1191 1271 1261 1276 1249 34.34 2.7% Sample 3 5795 5828 6134 6129 5971 160.43 2.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 303 331 321 324 319 10.32 3.2% Sample 1 303 331 321 324 319 10.32 3.2% Sample 2 1191 1271 1261 1276 1249 34.34 2.7% Sample 2 1191 1271 1261 1276 1249 34.34 2.7% Sample 3 5795 5828 6134 6129 5971 160.43 2.6% Sample 3 5795 5828 6134 6129 5971 160.43 2.6%

Synonyms

Interleukin-17 receptor B; IL-17 receptor B; IL-17RB; Cytokine receptor-like 4; IL-17 receptor homolog 1; IL-17Rh1; IL17Rh1; Interleukin-17B receptor; IL-17B receptor; IL17RB; CRL4, EVI27, IL17BR; UNQ2501/PRO19612

Gene Name

IL17RB

Gene ID

55540

UniProt

Q9NRM6

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: R18-K286

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Expressed in several endocrine tissues, mostly in fetal and adult liver, kidney, pancreas, testis, colon, brain and small intestine; not detected in peripheral blood leukocytes, lymphoid organs, and most cell lines.

Applications

ELISA

Field of Research

Cancer, Cell Biology, Cell Cycle, Cell Differentiation, Cytokines, Immunology, Innate Immunity, Interleukins, Macrophage/Inflammation

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 303 1191 57321 321 1173 5862 Standard deviation 14.54 47.64 411.44 17.01 58.65 527.58 CV (%) 4.8% 4% 7.1% 5.3% 5% 9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 303 1191 57321 321 1173 5862 Standard deviation 14.54 47.64 411.44 17.01 58.65 527.58 CV (%) 4.8% 4% 7.1% 5.3% 5% 9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 303 1191 57321 321 1173 5862 Mean (pg/ml) 303 1191 57321 321 1173 5862 Standard deviation 14.54 47.64 411.44 17.01 58.65 527.58 Standard deviation 14.54 47.64 411.44 17.01 58.65 527.58 CV (%) 4.8% 4% 7.1% 5.3% 5% 9% CV (%) 4.8% 4% 7.1% 5.3% 5% 9%

Sensitivity

<10 pg/mL

Function

Receptor for the proinflammatory cytokines IL17B and IL17E. May play a role in controlling the growth and/or differentiation of hematopoietic cells. .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

55885 MW

Specificity

Natural and recombinant human IL-17RB

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Interleukin-17 receptor B

Subcellular Location

Isoform 1: Cell membrane; Single-pass type I membrane protein.

Sequence Similarities

Contains 1 SEFIR domain.

Protein Name

Interleukin-17 receptor B

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.038 0.066 0.1 0.16 0.299 0.568 1.193 2.153 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.038 0.066 0.1 0.16 0.299 0.568 1.193 2.153 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.038 0.066 0.1 0.16 0.299 0.568 1.193 2.153 O.D. 0.038 0.066 0.1 0.16 0.299 0.568 1.193 2.153

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.