Human CRP/C Reactive Protein ELISA Kit PicoKine®

C Reactive Protein (CRP) is a major acute phase reactant synthesized primarily in the liver hepatocytes. It is composed of 5 identical, 21,500-molecular weight subunits. CRP mediates activities associated with preimmune nonspecific host resistance. CRP shows the strongest association with cardiovascular events. It is detectable on the surface of about 4% of normal peripheral blood lymphocytes. Acute phase reactant CRP is produced in the liver.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 593 514 598 593 574 34.98 6% Sample 2 1488 1753 1588 1771 1650 117.62 7.1% Sample 3 8142 7585 8200 7898 7956 242.45 3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 593 514 598 593 574 34.98 6% Sample 1 593 514 598 593 574 34.98 6% Sample 2 1488 1753 1588 1771 1650 117.62 7.1% Sample 2 1488 1753 1588 1771 1650 117.62 7.1% Sample 3 8142 7585 8200 7898 7956 242.45 3% Sample 3 8142 7585 8200 7898 7956 242.45 3%

Epigenetics and Nuclear Signaling, Metabolism, Immunology, Apoptosis, Atherosclerosis, Cardiovascular, Chromatin Binding Proteins, DNA/RNA Binding, Innate Immunity, Macrophage/Inflammation, Vascular Inflammation, Complement

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 593 1488 8142 565 1551 8792 Standard deviation 36.17 62.49 643.21 38.42 77.99 861.61 CV (%) 6.1% 4.2% 7.9% 6.8% 4.9% 9.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 593 1488 8142 565 1551 8792 Standard deviation 36.17 62.49 643.21 38.42 77.99 861.61 CV (%) 6.1% 4.2% 7.9% 6.8% 4.9% 9.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 593 1488 8142 565 1551 8792 Mean (pg/ml) 593 1488 8142 565 1551 8792 Standard deviation 36.17 62.49 643.21 38.42 77.99 861.61 Standard deviation 36.17 62.49 643.21 38.42 77.99 861.61 CV (%) 6.1% 4.2% 7.9% 6.8% 4.9% 9.8% CV (%) 6.1% 4.2% 7.9% 6.8% 4.9% 9.8%

Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.031 0.089 0.156 0.339 0.644 1.077 1.676 2.044 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.031 0.089 0.156 0.339 0.644 1.077 1.676 2.044 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.031 0.089 0.156 0.339 0.644 1.077 1.676 2.044 O.D. 0.031 0.089 0.156 0.339 0.644 1.077 1.676 2.044

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.