Mouse CD48/Slamf2 ELISA Kit PicoKine®

CD48 antigen (Cluster of Differentiation 48), also known as BLAST-1 or SLAMF2, is a protein that in humans is encoded by the CD48 gene. CD48 is a member of the CD2 subfamily of the immunoglobulin superfamily (IgSF) which includes SLAM (signaling lymphocyte activation molecules) proteins, such as CD84, CD150, CD229 and CD244. It is mapped to 1q23.3. CD48 is found on the surface of lymphocytes and other immune cells, dendritic cells and endothelial cells, and participates in activation and differentiation pathways in these cells. The encoded protein does not have a transmembrane domain, however, but is held at the cell surface by a GPI anchor via a C-terminal domain which maybe cleaved to yield a soluble form of the receptor.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 91 79 95 86 87 5.97 6.8% Sample 2 816 795 773 755 784 22.93 2.9% Sample 3 1332 1482 1540 1536 1472 84.28 5.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 91 79 95 86 87 5.97 6.8% Sample 1 91 79 95 86 87 5.97 6.8% Sample 2 816 795 773 755 784 22.93 2.9% Sample 2 816 795 773 755 784 22.93 2.9% Sample 3 1332 1482 1540 1536 1472 84.28 5.7% Sample 3 1332 1482 1540 1536 1472 84.28 5.7%

CD48 antigen; BCM1 surface antigen; BLAST-1; HM48-1; MRC OX-45 surface antigen; SLAM family member 2; SLAMF2; Signaling lymphocytic activation molecule 2; sgp-60; CD48; Cd48; Bcm-1

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 91 816 1332 95 736 1273 Standard deviation 4 34.27 87.91 5.03 41.21 98.02 CV (%) 4.4% 4.2% 6.6% 5.3% 5.6% 7.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 91 816 1332 95 736 1273 Standard deviation 4 34.27 87.91 5.03 41.21 98.02 CV (%) 4.4% 4.2% 6.6% 5.3% 5.6% 7.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 91 816 1332 95 736 1273 Mean (pg/ml) 91 816 1332 95 736 1273 Standard deviation 4 34.27 87.91 5.03 41.21 98.02 Standard deviation 4 34.27 87.91 5.03 41.21 98.02 CV (%) 4.4% 4.2% 6.6% 5.3% 5.6% 7.7% CV (%) 4.4% 4.2% 6.6% 5.3% 5.6% 7.7%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.034 0.072 0.114 0.189 0.385 0.705 1.262 1.969 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.034 0.072 0.114 0.189 0.385 0.705 1.262 1.969 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.034 0.072 0.114 0.189 0.385 0.705 1.262 1.969 O.D. 0.034 0.072 0.114 0.189 0.385 0.705 1.262 1.969

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.