Mouse CD70/TNFSF7/cd27L ELISA Kit PicoKine®

Mouse CD70/TNFSF7/cd27L ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Cd70 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

CD70 (Cluster of Differentiation 70), also known as TNFSF7 or CD27L, is a ligand for CD27. This gene is mapped to 19p13.3. CD70 is a surface antigen found on activated, but not resting, T and B lymphocytes. It has been found that CD70 induced proliferation of costimulated T cells and enhanced generation of cytolytic T cells. CD70 is homologous to the ligands of the TNF receptor family, including TNF-alpha, TNF-beta, and the CD40 ligand, showing 19 to 24% amino acid sequence identity in the extracellular region.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 79 82 85 75 80 3.69 4.6% Sample 2 248 239 232 248 241 6.72 2.7% Sample 3 791 821 875 932 854 53.8 6.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 79 82 85 75 80 3.69 4.6% Sample 1 79 82 85 75 80 3.69 4.6% Sample 2 248 239 232 248 241 6.72 2.7% Sample 2 248 239 232 248 241 6.72 2.7% Sample 3 791 821 875 932 854 53.8 6.2% Sample 3 791 821 875 932 854 53.8 6.2%

Synonyms

CD70 antigen; CD27 ligand; CD27-L; Tumor necrosis factor ligand superfamily member 7; CD70; Cd70; Cd27l, Cd27lg, Tnfsf7

Gene Name

CD70

Gene ID

21948

UniProt

O55237

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: S45-P195

Capture Antibody

Monoclonal antibody from rat

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Very low level of expression. Detected in splenocytes and thymocytes.

Applications

ELISA

Field of Research

Cancer, Cytokines, Growth Factors, Hematopoietic Progenitors, Immunology, Innate Immunity, Lymphoid, Stem Cells, T Lymphocytic Lineage, TNF Superfamily

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 79 248 791 80 240 839 Standard deviation 3.71 18.35 60.9 3.92 19.92 75.51 CV (%) 4.7% 7.4% 7.7% 4.9% 8.3% 9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 79 248 791 80 240 839 Standard deviation 3.71 18.35 60.9 3.92 19.92 75.51 CV (%) 4.7% 7.4% 7.7% 4.9% 8.3% 9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 79 248 791 80 240 839 Mean (pg/ml) 79 248 791 80 240 839 Standard deviation 3.71 18.35 60.9 3.92 19.92 75.51 Standard deviation 3.71 18.35 60.9 3.92 19.92 75.51 CV (%) 4.7% 7.4% 7.7% 4.9% 8.3% 9% CV (%) 4.7% 7.4% 7.7% 4.9% 8.3% 9%

Sensitivity

<10 pg/mL

Function

Cytokine that binds to CD27. Plays a role in T-cell activation. Induces the proliferation of costimulated T-cells and enhances the generation of cytolytic T-cells. .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

21919 MW

Specificity

Natural and recombinant mouse CD70

Anticoagulant

Heparin or EDTA

Gene Name Synonym

CD70 antigen

Subcellular Location

Membrane; Single-pass type II membrane protein.

Protein Name

CD70 antigen

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.063 0.142 0.206 0.315 0.514 0.785 1.327 2.188 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.063 0.142 0.206 0.315 0.514 0.785 1.327 2.188 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.063 0.142 0.206 0.315 0.514 0.785 1.327 2.188 O.D. 0.063 0.142 0.206 0.315 0.514 0.785 1.327 2.188

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.