Mouse CD70/TNFSF7/cd27L ELISA Kit PicoKine®

CD70 (Cluster of Differentiation 70), also known as TNFSF7 or CD27L, is a ligand for CD27. This gene is mapped to 19p13.3. CD70 is a surface antigen found on activated, but not resting, T and B lymphocytes. It has been found that CD70 induced proliferation of costimulated T cells and enhanced generation of cytolytic T cells. CD70 is homologous to the ligands of the TNF receptor family, including TNF-alpha, TNF-beta, and the CD40 ligand, showing 19 to 24% amino acid sequence identity in the extracellular region.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 79 82 85 75 80 3.69 4.6% Sample 2 248 239 232 248 241 6.72 2.7% Sample 3 791 821 875 932 854 53.8 6.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 79 82 85 75 80 3.69 4.6% Sample 1 79 82 85 75 80 3.69 4.6% Sample 2 248 239 232 248 241 6.72 2.7% Sample 2 248 239 232 248 241 6.72 2.7% Sample 3 791 821 875 932 854 53.8 6.2% Sample 3 791 821 875 932 854 53.8 6.2%

Cancer, Cytokines, Growth Factors, Hematopoietic Progenitors, Immunology, Innate Immunity, Lymphoid, Stem Cells, T Lymphocytic Lineage, TNF Superfamily

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 79 248 791 80 240 839 Standard deviation 3.71 18.35 60.9 3.92 19.92 75.51 CV (%) 4.7% 7.4% 7.7% 4.9% 8.3% 9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 79 248 791 80 240 839 Standard deviation 3.71 18.35 60.9 3.92 19.92 75.51 CV (%) 4.7% 7.4% 7.7% 4.9% 8.3% 9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 79 248 791 80 240 839 Mean (pg/ml) 79 248 791 80 240 839 Standard deviation 3.71 18.35 60.9 3.92 19.92 75.51 Standard deviation 3.71 18.35 60.9 3.92 19.92 75.51 CV (%) 4.7% 7.4% 7.7% 4.9% 8.3% 9% CV (%) 4.7% 7.4% 7.7% 4.9% 8.3% 9%

Cytokine that binds to CD27. Plays a role in T-cell activation. Induces the proliferation of costimulated T-cells and enhances the generation of cytolytic T-cells. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.063 0.142 0.206 0.315 0.514 0.785 1.327 2.188 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.063 0.142 0.206 0.315 0.514 0.785 1.327 2.188 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.063 0.142 0.206 0.315 0.514 0.785 1.327 2.188 O.D. 0.063 0.142 0.206 0.315 0.514 0.785 1.327 2.188

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.