Mouse TNFSF18/GITRL ELISA Kit PicoKine®

Tumor necrosis factor ligand superfamily member 18, also known as AITRL or GITRL, is a protein that in humans is encoded by the TNFSF18 gene. The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. It is mapped to 1q25.1. TNFSF18 is a ligand for receptor TNFRSF18/AITR/GITR. It has been shown to modulate T lymphocyte survival in peripheral tissues. This cytokine is also found to be expressed in endothelial cells, and is thought to be important for interaction between T lymphocytes and endothelial cells. TNFSF18-dependent modulation of tryptophan catabolism may represent an important mechanism of action of glucocorticoids, both physiologically and therapeutically.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 56 54 62 59 57 3.03 5.3% Sample 2 252 248 247 221 242 12.26 5% Sample 3 852 865 890 949 889 37.23 4.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 56 54 62 59 57 3.03 5.3% Sample 1 56 54 62 59 57 3.03 5.3% Sample 2 252 248 247 221 242 12.26 5% Sample 2 252 248 247 221 242 12.26 5% Sample 3 852 865 890 949 889 37.23 4.1% Sample 3 852 865 890 949 889 37.23 4.1%

Detected in immature and mature dendritic cells and in macrophages (at protein level) . Detected in spleen, lung, heart, thymus, monocytes, macrophages, B-cells and dendritic cells. .

Cancer, Cardiovascular, Cell Type Markers, Cytokines, Growth Factors, Growth Factors/Hormones, Immunology, Innate Immunity, Signal Transduction, Tags & Cell Markers, TNF Superfamily, Vasculature

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 56 252 852 60 250 921 Standard deviation 3.24 17.38 43.45 3.96 18.5 62.62 CV (%) 5.8% 6.9% 6.8%% 6.6% 7.4% 6.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 56 252 852 60 250 921 Standard deviation 3.24 17.38 43.45 3.96 18.5 62.62 CV (%) 5.8% 6.9% 6.8%% 6.6% 7.4% 6.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 56 252 852 60 250 921 Mean (pg/ml) 56 252 852 60 250 921 Standard deviation 3.24 17.38 43.45 3.96 18.5 62.62 Standard deviation 3.24 17.38 43.45 3.96 18.5 62.62 CV (%) 5.8% 6.9% 6.8%% 6.6% 7.4% 6.8% CV (%) 5.8% 6.9% 6.8%% 6.6% 7.4% 6.8%

Cytokine that binds to TNFRSF18/AITR/GITR (PubMed:14521928, PubMed:14647196) . Regulates T-cell responses (PubMed:14647196) . Can function as costimulator and lower the threshold for T-cell activation and T-cell proliferation (PubMed:14608036, PubMed:15128759) . Important for interactions between activated T-lymphocytes and endothelial cells. Mediates activation of NF-kappa-B (PubMed:14521928, PubMed:14647196, PubMed:18178614) . Triggers increased phosphorylation of STAT1 and up-regulates expression of VCAM1 and ICAM1 (By similarity) . Promotes leukocyte adhesion to endothelial cells (PubMed:23892569) . Regulates migration of monocytes from the splenic reservoir to sites of inflammation (PubMed:24107315) . .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.017 0.078 0.136 0.246 0.453 0.842 1.533 2.596 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.017 0.078 0.136 0.246 0.453 0.842 1.533 2.596 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.017 0.078 0.136 0.246 0.453 0.842 1.533 2.596 O.D. 0.017 0.078 0.136 0.246 0.453 0.842 1.533 2.596

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.