Mouse CD169/SIGLEC-1/Sialoadhesin ELISA Kit PicoKine®

SIGLEC-1, also known as Sialoadhesin or CD169, is a cell adhesion molecule found on the surface of certain cells of the immune system called macrophages. This gene is mapped to 20p13. It belongs to the immunoglobulin superfamily (IgSF) . Since sialoadhesin binds sialic acids with its N-terminal IgV-domain, it is also a member of the SIGLEC family. The localization and expression of SIGLEC-1 in humans has altered coincident with the evolutionary loss of Neu5Gc. SIGLEC-1-positive macrophages have a dual physiologic function. They act as innate 'flypaper' by preventing the systemic spread of lymph-borne pathogens and as critical gatekeepers at the lymph-tissue interface that facilitate the recognition of particulate antigens by B cells and initiate humoral immune responses.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 331 367 348 351 349 12.77 3.6% Sample 2 1301 1260 1214 1306 1270 37.05 2.9% Sample 3 5540 5164 5135 4886 5181 233.61 4.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 331 367 348 351 349 12.77 3.6% Sample 1 331 367 348 351 349 12.77 3.6% Sample 2 1301 1260 1214 1306 1270 37.05 2.9% Sample 2 1301 1260 1214 1306 1270 37.05 2.9% Sample 3 5540 5164 5135 4886 5181 233.61 4.5% Sample 3 5540 5164 5135 4886 5181 233.61 4.5%

Detected in lymph node in the subcapsular sinus, interfollicular regions, and T and B-cell boundary (at protein level) . Expressed by macrophages in various tissues. Highest expression in spleen and lymph node with lower amounts in lung, liver, bone marrow, heart and skin. No expression in thymus, kidney, brain or small intestine. .

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 331 1301 5540 351 1258 5216 Standard deviation 21.18 84.56 371.18 26.67 86.8 432.92 CV (%) 6.4% 6.5% 6.7% 7.6% 6.9% 8.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 331 1301 5540 351 1258 5216 Standard deviation 21.18 84.56 371.18 26.67 86.8 432.92 CV (%) 6.4% 6.5% 6.7% 7.6% 6.9% 8.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 331 1301 5540 351 1258 5216 Mean (pg/ml) 331 1301 5540 351 1258 5216 Standard deviation 21.18 84.56 371.18 26.67 86.8 432.92 Standard deviation 21.18 84.56 371.18 26.67 86.8 432.92 CV (%) 6.4% 6.5% 6.7% 7.6% 6.9% 8.3% CV (%) 6.4% 6.5% 6.7% 7.6% 6.9% 8.3%

Acts as an endocytic receptor mediating clathrin dependent endocytosis. Macrophage-restricted adhesion molecule that mediates sialic-acid dependent binding to lymphocytes, including granulocytes, monocytes, natural killer cells, B-cells and CD8 T-cells (By similarity) . Preferentially binds to alpha- 2,3-linked sialic acid. Binds to SPN/CD43 on T-cells. May play a role in hematopoiesis. May act as a counter-receptor for CLEC10A in lymph node. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.013 0.062 0.125 0.223 0.465 0.841 1.547 2.216 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.013 0.062 0.125 0.223 0.465 0.841 1.547 2.216 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.013 0.062 0.125 0.223 0.465 0.841 1.547 2.216 O.D. 0.013 0.062 0.125 0.223 0.465 0.841 1.547 2.216

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.