Human CD169/SIGLEC-1/Sialoadhesin ELISA Kit PicoKine®

Human CD169/SIGLEC-1 ELISA Kit PicoKine® (96 Tests) . Quantitate Human SIGLEC1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

SIGLEC-1, also known as Sialoadhesin or CD169, is a cell adhesion molecule found on the surface of certain cells of the immune system called macrophages. This gene is mapped to 20p13. It belongs to the immunoglobulin superfamily (IgSF) . Since sialoadhesin binds sialic acids with its N-terminal IgV-domain, it is also a member of the SIGLEC family. The localization and expression of SIGLEC-1 in humans has altered coincident with the evolutionary loss of Neu5Gc. SIGLEC-1-positive macrophages have a dual physiologic function. They act as innate 'flypaper' by preventing the systemic spread of lymph-borne pathogens and as critical gatekeepers at the lymph-tissue interface that facilitate the recognition of particulate antigens by B cells and initiate humoral immune responses.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 278 276 287 263 276 8.57 3.1% Sample 2 1821 2107 2081 1805 1953 140.91 7.2% Sample 3 4073 4478 4741 4142 4358 268.77 6.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 278 276 287 263 276 8.57 3.1% Sample 1 278 276 287 263 276 8.57 3.1% Sample 2 1821 2107 2081 1805 1953 140.91 7.2% Sample 2 1821 2107 2081 1805 1953 140.91 7.2% Sample 3 4073 4478 4741 4142 4358 268.77 6.1% Sample 3 4073 4478 4741 4142 4358 268.77 6.1%

Synonyms

Sialoadhesin; Sialic acid-binding Ig-like lectin 1; Siglec-1; CD169; SIGLEC1; SN

Gene Name

SIGLEC1

Gene ID

6614

UniProt

Q9BZZ2

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: S20-Q1641

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Expressed by macrophages in various tissues. High levels are found in spleen, lymph node, perivascular macrophages in brain and lower levels in bone marrow, liver Kupffer cells and lamina propria of colon and lung. Also expressed by inflammatory macrophages in rheumatoid arthritis.

Applications

ELISA

Field of Research

Cell Type Markers, Immunology, Innate Immunity, Macrophage/Inflammation, Tags & Cell Markers

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 278 1821 4073 286 1650 3922 Standard deviation 20.01 138.39 240.3 22.59 148.5 262.77 CV (%) 7.2% 7.6% 5.9% 7.9% 9% 6.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 278 1821 4073 286 1650 3922 Standard deviation 20.01 138.39 240.3 22.59 148.5 262.77 CV (%) 7.2% 7.6% 5.9% 7.9% 9% 6.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 278 1821 4073 286 1650 3922 Mean (pg/ml) 278 1821 4073 286 1650 3922 Standard deviation 20.01 138.39 240.3 22.59 148.5 262.77 Standard deviation 20.01 138.39 240.3 22.59 148.5 262.77 CV (%) 7.2% 7.6% 5.9% 7.9% 9% 6.7% CV (%) 7.2% 7.6% 5.9% 7.9% 9% 6.7%

Sensitivity

<10 pg/mL

Function

Acts as an endocytic receptor mediating clathrin dependent endocytosis. Macrophage-restricted adhesion molecule that mediates sialic-acid dependent binding to lymphocytes, including granulocytes, monocytes, natural killer cells, B-cells and CD8 T-cells. Preferentially binds to alpha-2,3-linked sialic acid (By similarity) . Binds to SPN/CD43 on T-cells (By similarity) . May play a role in hemopoiesis. .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

182624 MW

Specificity

Natural and recombinant human CD169

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Sialoadhesin

Subcellular Location

Isoform 1: Cell membrane; Single-pass type I membrane protein.

Sequence Similarities

Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.

Protein Name

Sialoadhesin

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.019 0.095 0.153 0.285 0.507 0.96 1.669 2.326 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.019 0.095 0.153 0.285 0.507 0.96 1.669 2.326 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.019 0.095 0.153 0.285 0.507 0.96 1.669 2.326 O.D. 0.019 0.095 0.153 0.285 0.507 0.96 1.669 2.326

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.